具有维持造血干/祖细胞能力的新型豆类凝集素的分离、纯化及功能鉴定

一种分离自豆类的新型凝集素不仅具有凝集活性，还具有体外长期维持造血干/祖细胞的能力.由眉豆（Dolichos lablab）中分离得到了这种多亚基的凝集素——FRIL（Flt3 receptor-interacting lectin），并对它进行了核酸和蛋白质序列分析.免疫细胞分析显示，它的受体是CD34⁺造血干/祖细胞所特有的.在培养基中添加这种凝集素可长期维持CD34⁺细胞存活和增殖能力.以Flt3配基（FL）作为对照，在28天的培养时间内，相对于FL，FRIL可维持细胞较高的G0/G1期比例（80%以上G0/G1期）和长期培养中（14天以上）1.5倍以上的集落形成量.可见FRIL通过滞留造血干/祖细胞于G0/G1期而维持它们的自我更新潜能.

Extraction, Purification and Function Analysis of a New Lectin Which Can Maintain The Multipotential of Hematopoietic Stem/progenitor Cells In vitro

A new lectin extracted from lablab can maintain the multipotential of hematopoietic stem/progenitor cells in vitro for a long time in addition to its hemagglutination property. This new lectin FRIL (Flt3-rectptor interacting lectin) was purified from Dolichos lablab, its biological speciality was identified and its receptors were located on hematopoietic stem/progenitor cells. When CD34⁺ cells were cultured in the presence of this lectin or its counterpart FL, within the 28 days of culture, FRIL can keep more cells in G0/G1 phase (beyond 80%) and 1.5 fold colony forming cells more than FL does after 14-day's culture. So, it is concluded that FRIL maintain HSC/HPCs self-renewal property by halting its cell cycles.