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新疆维吾尔族妇女宫颈癌组织中乳头瘤病毒16型E6基因的克隆和序列分析
引用本文:马正海,钱东,马纪,林仁勇,闻明,钟哲,张富春,张秋云.新疆维吾尔族妇女宫颈癌组织中乳头瘤病毒16型E6基因的克隆和序列分析[J].生物化学与生物物理进展,2001,28(3):400-404.
作者姓名:马正海  钱东  马纪  林仁勇  闻明  钟哲  张富春  张秋云
作者单位:1. 新疆大学生物系分子生物学重点实验室,
2. 乌鲁木齐市医学研究所,
基金项目:国家自然科学基金资助项目(39960079).
摘    要:为了分析新疆南部地区维吾尔族妇女宫颈癌组织中HPV16型E6基因结构特点,从中国新疆南部地区维吾尔族妇女宫颈癌活检组织标本中提取DNA,以宫颈癌活检组织标本DNA为模板进行PCR扩增,获得HPV16 E6基因,将其克隆到pUCm-T载体上,并对其进行基因全序列分析.PCR检测结果显示宫颈癌组织中HPV16 E6阳性率为82.35%(14/17);测序结果显示,新疆株HPV16 E6基因全长456 bp,大小与德国标准株一致.E6基因的第247位碱基发生T→G突变,并由此引起所编码的氨基酸亦发生改变.上述结果表明,中国新疆南部地区维吾尔族妇女宫颈癌患者组织中HPV16 E6的基因结构与德国标准株HPV16 E6基因之间存在差异.

关 键 词:人乳头瘤病毒16型,宫颈癌,E6基因,核苷酸序列分析
收稿时间:7/7/2000 12:00:00 AM
修稿时间:2000/9/28 0:00:00

Cloning and Sequencing of HPV16 E6 Gene from Cervical Carcinoma Biopsies in Xinjiang
MA Zheng-Hai,QIAN Dong,MA Ji,LIN Ren-Yong,WEN Ming,ZHONG Zhe,ZHANG Fu-Chun and ZHANG Qiu-Yun.Cloning and Sequencing of HPV16 E6 Gene from Cervical Carcinoma Biopsies in Xinjiang[J].Progress In Biochemistry and Biophysics,2001,28(3):400-404.
Authors:MA Zheng-Hai  QIAN Dong  MA Ji  LIN Ren-Yong  WEN Ming  ZHONG Zhe  ZHANG Fu-Chun and ZHANG Qiu-Yun
Institution:Key Laboratory of Molecular Biology, Department of Biology, Xinjiang University, Urumuqi 830046, China;Key Laboratory of Molecular Biology, Department of Biology, Xinjiang University, Urumuqi 830046, China;Key Laboratory of Molecular Biology, Department of Biology, Xinjiang University, Urumuqi 830046, China;Key Laboratory of Molecular Biology, Department of Biology, Xinjiang University, Urumuqi 830046, China;Key Laboratory of Molecular Biology, Department of Biology, Xinjiang University, Urumuqi 830046, China;Key Laboratory of Molecular Biology, Department of Biology, Xinjiang University, Urumuqi 830046, China;Key Laboratory of Molecular Biology, Department of Biology, Xinjiang University, Urumuqi 830046, China;Institute of Urumuqi Medicine, Urumuqi, Xinjiang 830010, China
Abstract:In order to study the structure specificity of HPV16 E6 gene of a Chinese Uygur patient of cervical carcinoma in south Xinjiang, the tissue DNA was extracted from cervical carcinoma biopsies. HPV16E6 gene was amplified by PCR from the cervical carcinoma tissue DNA. The HPV16 E6 gene was cloned into pUCm-T and analyzed the whole sequence. The result of PCR showed that the positive rate of HPV16 E6 was 82.35%(14/17). The result of sequence showed that the overall length of strain is 456 bp, it was the same as the German strain. The 247th nucleotide of HPV16 E6 mutate T to G, the mutation had changed the triplet codes, subsequently changed the amino acids coded. There is a structure difference between HPV16 E6 gene of Xinjiang strain and the standard strain.
Keywords:human papillomavirus type16  cervical carcinoma  E6 Gene  DNA sequencing
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