神经生长因子介导的促神经生长化合物筛选系统的建立

为了合理评价化合物的促神经再生活性.用溴化四唑蓝(MTT)、分化计数、图象处理等方法，借助FK506、GPI1046阳性化合物，建立了一个基于PC12细胞存活和分化的化合物筛选系统.结果表明，无论在细胞存活实验还是在分化实验中，FK506、GPI1046都可以明显增强神经生长因子(NGF)的效应，即有促神经再生的作用.也就是说，这一系统将有助于从组合化学方法合成的化合物文库中，筛选出具有促神经再生活性的化合物.

Screening System for Nerve Growth Factor (NGF)-mediated Neurotrophic Compounds

To evaluate reasonably the neurotrophic action of small molecule. MTT, differentiation quantity and image analysis methods were used, with the FK506 and GPI1046 as positive control chemicals. A PC12 cell survival and differentiation based screening system were established, in which FK506 and GPI1046 sharply enhanced the neurotrophic action of NGF both in survival and differentiation model just as expected. The system will be helpful in screening neurotrophic novel compounds from chemical library produced by combinatorial chemistry.