| Pb~(2+)、Cd~(2+)和Ce~(3+)对猪胰α-淀粉酶活性的影响 |
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| 引用本文： | 洪法水,王雪峰,沈颂东,苏国兴,潘新法.Pb~(2+)、Cd~(2+)和Ce~(3+)对猪胰α-淀粉酶活性的影响[J].生物化学与生物物理进展,2002,29(1). |
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| 作者姓名： | 洪法水 王雪峰 沈颂东 苏国兴 潘新法 |
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| 作者单位： | 苏州大学生物科学系,苏州,215006 |
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| 基金项目： | 苏州大学“2 1 1工程”科研基金资助项目(XQ31 60 1 1 )~~ |
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| 摘 要： | 分别研究了Pb2 + 、Cd2 + 和Ce3 + 对Ca (Ⅱ )α 淀粉酶活性影响及对其Ca2 + 的竞争作用 .结果表明三种金属离子低浓度情况下 (0 5～ 5mmol/L)对α 淀粉酶具有激活现象 ,而较高浓度则抑制酶活力 .Pb2 + 、Cd2 + 和Ce3 + 竞争置换α 淀粉酶中Ca2 + 能力的大小是 :Pb2 + >Cd2 + >Ce3 + ,其抑制酶活作用大小 :Pb2 + >Cd2 + >Ce3 + .
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| 关 键 词： | α-淀粉酶 重金属离子 抑制常数 竞争结合 |
Effects of metal ions Ce3+,Cd2+ and Pb2+ on the activity of α-amylase from porcine pancreas |
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| HONG Fa-Shui ,WANG Xue-Feng,SHEN Song-Dong,SU Guo-Xing,PAN Xin-Fa.Effects of metal ions Ce3+,Cd2+ and Pb2+ on the activity of α-amylase from porcine pancreas[J].Progress In Biochemistry and Biophysics,2002,29(1). |
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| Authors: | HONG Fa-Shui WANG Xue-Feng SHEN Song-Dong SU Guo-Xing PAN Xin-Fa |
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| Institution: | HONG Fa-Shui **,WANG Xue-Feng,SHEN Song-Dong,SU Guo-Xing,PAN Xin-Fa |
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| Abstract: | Ce 3+,Cd 2+ and Pb 2+ could influence the activity of amylase from porcine pancreas and competitively replace Ca 2+ from amylase. The results showed that the activity of amylase was enhanced under the treatment of Ce 3+,Cd 2+ and Pb 2+ respectively at low concerntration, but inhibited at the high concerntration. Both replacement and inhibition showed that the action was in the order: Pb 2+>Cd 2+>Ce 3+. |
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| Keywords: | amylase heavy metal ions inhibition constant competitively combine |
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