| 梨形环棱螺凝集素的初步研究 |
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| 引用本文： | 张吉强,李清漪.梨形环棱螺凝集素的初步研究[J].生物化学与生物物理进展,1995,22(3):281-283. |
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| 作者姓名： | 张吉强 李清漪 |
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| 作者单位： | 第三军医大学组织胚胎学教研室;西南师范大学生物系 |
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| 摘 要： | 通过Sepharose 4B-甲状腺球蛋白亲和层析,从梨形环棱螺Bellamya purificata体内分离到的一种凝集素,不连续PAGE显示其为单一的蛋白质谱带.它能凝集兔、猪、鸭等动物的红细胞,但不能凝集人的A、B、O及AB型血的红细胞和固定后的兔红细胞.其凝集活力可被1.0mol/L的乳糖、半乳糖和60g/L的甲状腺球蛋白抑制,但不能被碱性硼酸缓冲液抑制.对温度变化敏感,有较宽的最适pH范围.
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| 关 键 词： | 梨形环棱螺 凝集素 理化性质 纯化 |
| 收稿时间： | 1994/4/25 0:00:00 |
| 修稿时间： | 1994/7/16 0:00:00 |
A Preliminary Study on Bellamy purificata Lectin |
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| Zhang Jiqiang and Li Qingyi.A Preliminary Study on Bellamy purificata Lectin[J].Progress In Biochemistry and Biophysics,1995,22(3):281-283. |
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| Authors: | Zhang Jiqiang and Li Qingyi |
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| Institution: | Third Military Medical College;Third Military Medical College |
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| Abstract: | By Sepharose 4B-thyroglobulin affinity column chromatography a lectin was purified from Bellamya purificata. It showed a single band on alkaline PAGE. This lectin (BPL) agglutinated red blood cells of rabbit,duck and pig,but not of human blood types or formalin and/or glutaraldehyde-fixed rabbit erythrocytes. Its agglutination activity could be inhibited by 1.0mol/L lactose or galactose as well as 60g/L thyroglobulin, but not by alkaline borate buffer.BPL was temperature-sensitive,while showed a broad optical pH range (pH4.56-9.96). |
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| Keywords: | Bellamya purificata lectin physicochemical characterization purification |
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