| hTERT启动子调控rVBMDMP基因表达载体的构建与功能研究 |
| |
| 引用本文： | 张志伟,贺修胜,罗招阳,李艳兰,曹建国.hTERT启动子调控rVBMDMP基因表达载体的构建与功能研究[J].生物化学与生物物理进展,2010,37(4):416-425. |
| |
| 作者姓名： | 张志伟 贺修胜 罗招阳 李艳兰 曹建国 |
| |
| 作者单位： | 南华大学医学院肿瘤研究所;南华大学医学院病理学教研室;南华大学肿瘤细胞与分子病理学湖南省高校重点实验室;湖南师范大学医学院药理学教研室; |
| |
| 基金项目： | 湖南省自然科学基金(07JJ6164); 湖南省重点学科建设基金(2006-180)资助项目~~ |
| |
| 摘 要： | 为了探讨端粒酶催化亚单位(hTERT)启动子调控重组血管基膜衍生多功能肽(rVBMDMP)基因表达抑制肺癌细胞生长的作用机制,采用PCR方法,克隆hTERT启动子核心区片段,并检测其功能活性.然后,构建pLNSX/hTERT/rVBMDMP逆病毒载体,获取逆病毒,感染肺癌A549细胞,检测hTERT启动子调控rVBMDMP基因表达对细胞形态、细胞生长、细胞凋亡以及Caspase-3表达的影响.另外,观察hTERT启动子调控rVBMDMP基因表达对裸鼠成瘤的抑制作用、瘤组织细胞的凋亡及Caspase-3表达情况.结果发现:a.hTERT启动子核心区能调控rVBMDMP基因的表达(n=3,P<0.05);b.hTERT启动子调控rVBMDMP基因的表达具有抑制肺癌A549细胞的生长和裸鼠成瘤作用(n=6,P<0.05);c.rVBMDMP基因表达能促进肺癌A549细胞凋亡和Caspase-3蛋白表达.以上结果说明,hTERT启动子调控rVBMDMP基因表达后,通过提高Caspase-3的表达水平,促进细胞凋亡而抑制肺癌A549细胞的生长.
|
| 关 键 词： | hTERT启动子 rVBMDMP基因 细胞凋亡 Caspase-3蛋白 |
| 收稿时间： | 2009/8/24 0:00:00 |
| 修稿时间： | 2010/1/24 0:00:00 |
Construction and Function Study of Expression Vector of rVBMDMP Gene Regulated by hTERT Promotor |
| |
| ZHANG Zhi-Wei,HE Xiu-Sheng,LUO Zhao-Yang,LI Yan-Lan and CAO Jian-Guo.Construction and Function Study of Expression Vector of rVBMDMP Gene Regulated by hTERT Promotor[J].Progress In Biochemistry and Biophysics,2010,37(4):416-425. |
| |
| Authors: | ZHANG Zhi-Wei HE Xiu-Sheng LUO Zhao-Yang LI Yan-Lan and CAO Jian-Guo |
| |
| Institution: | ZHANG Zhi-Wei1,2,3)**,HE Xiu-Sheng1,3),LUO Zhao-Yang1,LI Yan-Lan1,CAO Jian-Guo1,4) (1) Cancer Research Institute of Medical College,University of Southern China,Hengyang 421001,China,2) Department of Pathology of Medical College,3) University Key Laboratory of Cancer Cellular , Molecular Pathology in Hunan Province,4) Department of Pharmacology of Medical College,Hunan Normal University,Changsha 410013,C... |
| |
| Abstract: | To investigate action mechanism of the expression of rVBMDMP gene regulated by hTERT promotor inhibited cell growth of lung cancer, the Core domain of hTERT promotor was obtained by PCR and its function activity was detected. Then, retroviral vector of pLNSX/hTERT/rVBMDMP was recombined, and lung cancer A549 cells were infected by the collected vetrovirus. Further, it was observed that expression of rVBMDMP gene regulated by hTERT promotor influenced cellular morphous, cell growth, cell apoptosis and expres... |
| |
| Keywords: | hTERT promotor rVBMDMP gene cell apoptosis Caspase-3 protein |
| 本文献已被 CNKI 等数据库收录！ |
| 点击此处可从《生物化学与生物物理进展》浏览原始摘要信息 |
| 点击此处可从《生物化学与生物物理进展》下载免费的PDF全文 |
|
