| 肺炎克雷伯菌荚膜糖蛋白ELISA检测方法的研究 |
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| 引用本文： | 李轻舟,张燕,戚薇,杜连祥.肺炎克雷伯菌荚膜糖蛋白ELISA检测方法的研究[J].生物化学与生物物理进展,2004,31(6):567-571. |
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| 作者姓名： | 李轻舟 张燕 戚薇 杜连祥 |
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| 作者单位： | 天津科技大学食品与生物工程学院,天津,300222 |
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| 摘 要： | 以肺炎克雷伯菌荚膜糖蛋白为免疫原，获得了兔抗肺炎克雷伯菌荚膜糖蛋白抗血清，通过硫酸铵分级沉淀与吸附法相结合的方法对抗血清进行纯化，并在此基础上建立了肺炎克雷伯菌荚膜糖蛋白间接ELISA检测方法.该检测方法的检测灵敏度为0.031 mg/L，线性检测范围为2.5～30.0 mg/L，批内误差为1.04％～3.22％，批间误差为2.61％～8.35％.
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| 关 键 词： | 肺炎克雷伯菌荚膜糖蛋白，间接ELISA，检测方法 |
| 收稿时间： | 2003/11/24 0:00:00 |
| 修稿时间： | 2004/2/28 0:00:00 |
The Enzyme-linked Immunosorbent Assay of Klebsiella pneumoniae Capsule Glycoprotein |
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| LI Qing-Zhou,ZHANG Yan,QI Wei and DU Lian-Xiang.The Enzyme-linked Immunosorbent Assay of Klebsiella pneumoniae Capsule Glycoprotein[J].Progress In Biochemistry and Biophysics,2004,31(6):567-571. |
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| Authors: | LI Qing-Zhou ZHANG Yan QI Wei and DU Lian-Xiang |
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| Institution: | Food and Biotechnology Engineering Department, Tianjin University of Science and Technology, Tianjin 300222, China;Food and Biotechnology Engineering Department, Tianjin University of Science and Technology, Tianjin 300222, China;Food and Biotechnology Engineering Department, Tianjin University of Science and Technology, Tianjin 300222, China;Food and Biotechnology Engineering Department, Tianjin University of Science and Technology, Tianjin 300222, China |
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| Abstract: | An indirect enzyme-linked immunosorbent assay (ELISA) was developed to detect and quantitate the Klebsiella pneumoniae (Kp) capsule glycoprotein. The rabbit antiserum of Klebsiella pneumoniae capsule glycoprotein was prepared successfully by using a purified Kp capsule glycoprotein as antigen and the antibody was purified with ammonium sulfate fractionation and adsorption. The sensitivity of the assay is 0.031 mg/L. The standard curve is linear from 2.5 mg/L to 30 mg/L. The error of inter-plate is from 1.04% to 3.22%, and the error of intra-plate is from 2.61% to 8.35%. |
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| Keywords: | Klebsiella pneumoniae capsule glycoprotein indirect enzyme-linked immunosorbent assay mensuration |
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