| 植物炭疽病真菌金属硫蛋白的三级结构预测 |
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| 引用本文： | 何红珍,吕暾,赵南明,张日清,刘进元.植物炭疽病真菌金属硫蛋白的三级结构预测[J].生物化学与生物物理进展,2002,29(2):293-296. |
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| 作者姓名： | 何红珍 吕暾 赵南明 张日清 刘进元 |
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| 作者单位： | 清华大学生物科学与技术系,生物膜与膜生物工程国家重点实验室,北京 100084 |
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| 基金项目： | 国家高技术863青年基金，教育部跨世纪人才基金和国家重大基础研究（G19998010100）资助项目. |
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| 摘 要： | 根据实验测定的Ⅰ类金属硫蛋白(metallothionein, MT)三级结构的实验数据，给出该类蛋白质的两种特征结构(CXC、CXXC一级结构，半胱氨酸-金属络合簇三级结构)的原子间距离约束条件，然后运用距离几何算法计算出一系列可能的构象.从这些构象中经统计分析筛选出目标函数值显著较小的结构作为所预测蛋白质的三级结构模型.用已知结构的蓝蟹MT对方法进行检验证实其可行性后，对植物炭疽病真菌金属硫蛋白CAP3进行了三级结构预测.
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| 关 键 词： | 金属硫蛋白CAP3，三级结构预测，距离几何算法 |
| 收稿时间： | 2001/7/30 0:00:00 |
| 修稿时间： | 2001年7月30日 |
A Prediction for The Tertiary Structure of Metallothionein CAP3 From Colletotrichum gloeosporioides |
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| Institution: | Department of Biological Science and Biotechnology, Tsinghua University, State Key Laboratory of Biomembrane and Biotechnology, Beijing 100084, China;Department of Biological Science and Biotechnology, Tsinghua University, State Key Laboratory of Biomembrane and Biotechnology, Beijing 100084, China;Department of Biological Science and Biotechnology, Tsinghua University, State Key Laboratory of Biomembrane and Biotechnology, Beijing 100084, China;Department of Biological Science and Biotechnology, Tsinghua University, State Key Laboratory of Biomembrane and Biotechnology, Beijing 100084, China;Department of Biological Science and Biotechnology, Tsinghua University, State Key Laboratory of Biomembrane and Biotechnology, Beijing 100084, China |
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| Abstract: | Based on the experimental data of metallothionein-I (MT-I) tertiary structures, interatomic distance constraints of two characteristic structures (CXC, CXXC sequence pattern and metal-Cys chelate tertiary structure) were constructed. Then the distance geometry method was used to work out a number of possible structures, from which those with remarkably lower target function value were selected by a statistical analysis as the predicted tertiary structure models. The predicted structure of blue crab by this method was similar to the experimentally determined structure showed that this method could be used to MT prediction. The tertiary structure of a metallothionein CAP3 from Colletotrichum gloeosporioides was modeled. The predicted structure is similar to those of its homologous MT proteins in metal-Cys combination, and reasonable in its structure energy as well as in its fold motif. |
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| Keywords: | metallothionein CAP3 tertiary structure prediction distance geometry method |
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