| 一种简易的免疫PCR方法的建立 |
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| 引用本文： | 徐平西,肖华胜,鞠躬.一种简易的免疫PCR方法的建立[J].生物化学与生物物理进展,1999,26(4):396-398. |
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| 作者姓名： | 徐平西 肖华胜 鞠躬 |
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| 作者单位： | 第四军医大学神经科学研究所,西安,710032 |
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| 摘 要： | 免疫PCR为一种高敏感度检测抗原的新技术，操作程序大多沿袭ELISA方法.用戊二醛作连接剂，将蛋白质高效率包被在普通PCR管内壁，使免疫及PCR反应用普通PCR仪得以在管中连贯地进行.实验结果表明，标准曲线的线性关系好，与ELISA方法比较敏感度高出约105.这一改良法的建立，可望促进免疫PCR的普及应用.
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| 关 键 词： | 免疫PCR，包被，戊二醛，PCR管 |
| 收稿时间： | 1998/6/19 0:00:00 |
| 修稿时间： | : 1998-06- |
A Simplification of the Immuno-PCR Assay |
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| XU Ping-Xi,XIAO Hua-Sheng and JU Gong.A Simplification of the Immuno-PCR Assay[J].Progress In Biochemistry and Biophysics,1999,26(4):396-398. |
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| Authors: | XU Ping-Xi XIAO Hua-Sheng and JU Gong |
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| Institution: | Institute of Neurosciences,The Fourth Military Medical University, Xi'an 710032, China;Institute of Neurosciences,The Fourth Military Medical University, Xi'an 710032, China;Institute of Neurosciences,The Fourth Military Medical University, Xi'an 710032, China |
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| Abstract: | The Immuno-PCR antigen detection system has been developed for several years as a modefication of the ELISA method. But only a few of laboratorise applied this high sensitive quantitative assay, since it needs special apparutus or complicated operations.A new procedure of Immuno-PCR was established, which coats antibody or antigien on PCR tubes with 0.8% glutaraldehyde instead of on the wells of titer plates with alkine carbonate buffer. The assay could be coducted with simple handlings and ordinary apparutus, and a high sensitivity is obtained up to 105 times higher than that of ELISA in S-100 antigen detection. |
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| Keywords: | immuno-polymerase chain reaction(Immuno-PCR) coating glutaraldehyde PCR tube |
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