| 一个用于转染细胞分选的双顺反子载体的构建及其应用 |
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| 引用本文： | 曲笑霞,秦立蓬,岳 文,高艳红,李艳华,袁红丰,王韫芳,刘大庆,闫 舫,施双双,裴雪涛.一个用于转染细胞分选的双顺反子载体的构建及其应用[J].生物化学与生物物理进展,2005,32(9):889-894. |
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| 作者姓名： | 曲笑霞 秦立蓬 岳 文 高艳红 李艳华 袁红丰 王韫芳 刘大庆 闫 舫 施双双 裴雪涛 |
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| 作者单位： | 军事医学科学院输血研究所,干细胞研究室,北京,100850 |
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| 基金项目： | 国家高技术“863”计划资助项目（2002AA205050和2003AA205160），国家重点基础研究发展规划项目（973）（2001CB509906）和北京市科委自然科学基金资助项目（H020220010190）. |
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| 摘 要： | 为简化转染细胞的分选过程，构建了一个含有细胞表面标志 CD34 基因的双顺反子载体 p3.1-IRES-CD34. 利用来源于脑心肌炎病毒 (EMCV) 的内部核糖体进入位点 (IRES) ，实现目的基因与 CD34 基因的共同表达 . 将绿色荧光蛋白 (EGFP) 作为目的基因插入载体的多克隆位点，然后转染 NIH-3T3 细胞，通过免疫磁珠分选 (MACS) 方法来分选细胞 . 结果表明：对于转染细胞，均可实现快速分选 ( 瞬时转染细胞约 48 h ，稳定转染 10~15 天 ) ，并且获得较高纯度 (95% 以上 ) 的表达目的基因细胞 .
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| 关 键 词： | 载体，转染细胞，分选， CD34 |
| 收稿时间： | 04 7 2005 12:00AM |
| 修稿时间： | 2005-04-072005-05-27 |
A Bicistronic Expression Vector for The Selection of Transfected Cells |
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| QU Xiao-Xi,QIN Li-Peng,YUE Wen,GAO Yan-Hong,LI Yan-Hu,YUAN Hong-Feng,WANG Yun-Fang,LIU Da-Qing,YAN Fang,SHI Shuang-Shuang and PEI Xue-Tao.A Bicistronic Expression Vector for The Selection of Transfected Cells[J].Progress In Biochemistry and Biophysics,2005,32(9):889-894. |
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| Authors: | QU Xiao-Xi QIN Li-Peng YUE Wen GAO Yan-Hong LI Yan-Hu YUAN Hong-Feng WANG Yun-Fang LIU Da-Qing YAN Fang SHI Shuang-Shuang and PEI Xue-Tao |
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| Institution: | Laboratory of Stem Cell Biology, Beijing Institute of Transfusion Medicine, Military Medical Academy of Science, Beijing 100850, China;Laboratory of Stem Cell Biology, Beijing Institute of Transfusion Medicine, Military Medical Academy of Science, Beijing 100850, China;Laboratory of Stem Cell Biology, Beijing Institute of Transfusion Medicine, Military Medical Academy of Science, Beijing 100850, China;Laboratory of Stem Cell Biology, Beijing Institute of Transfusion Medicine, Military Medical Academy of Science, Beijing 100850, China;Laboratory of Stem Cell Biology, Beijing Institute of Transfusion Medicine, Military Medical Academy of Science, Beijing 100850, China;Laboratory of Stem Cell Biology, Beijing Institute of Transfusion Medicine, Military Medical Academy of Science, Beijing 100850, China;Laboratory of Stem Cell Biology, Beijing Institute of Transfusion Medicine, Military Medical Academy of Science, Beijing 100850, China;Laboratory of Stem Cell Biology, Beijing Institute of Transfusion Medicine, Military Medical Academy of Science, Beijing 100850, China;Laboratory of Stem Cell Biology, Beijing Institute of Transfusion Medicine, Military Medical Academy of Science, Beijing 100850, China;Laboratory of Stem Cell Biology, Beijing Institute of Transfusion Medicine, Military Medical Academy of Science, Beijing 100850, China;Laboratory of Stem Cell Biology, Beijing Institute of Transfusion Medicine, Military Medical Academy of Science, Beijing 100850, China |
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| Abstract: | |
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| Keywords: | vector transfected cells selection CD34 |
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