| 蛋白质溶液NMR结构测定的一些新进展 |
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| 引用本文： | 高广华,王大成.蛋白质溶液NMR结构测定的一些新进展[J].生物化学与生物物理进展,1999,26(3):228-233. |
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| 作者姓名： | 高广华 王大成 |
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| 作者单位： | 中国科学院生物物理研究所,北京,100101 |
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| 摘 要： | 新的标记技术的进展和采用稀释的液晶作为溶剂以提供额外的结构信息，提高了核磁共振技术测定蛋白质溶液三维结构的精度，扩大了分子质量测定范围.目前已经利用多维 15N,13C,2H标记NMR测定了许多分子质量为30 ku左右的蛋白质溶液结构，这一上限可能还会被进一步提高.
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| 关 键 词： | 核磁共振 蛋白质三维结构 液晶 偶极偶合 同位素标记 |
| 收稿时间： | 1998/2/24 0:00:00 |
| 修稿时间： | 6/4/1998 12:00:00 AM |
Progress in the Determination of Protein Solution Structure by NMR |
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| GAO Guang-Hua and WANG Da-Cheng.Progress in the Determination of Protein Solution Structure by NMR[J].Progress In Biochemistry and Biophysics,1999,26(3):228-233. |
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| Authors: | GAO Guang-Hua and WANG Da-Cheng |
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| Institution: | Institute of Biophysics, The Chinese Academy of Sciences, Beijing 100101, China;Institute of Biophysics, The Chinese Academy of Sciences, Beijing 100101, China |
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| Abstract: | Advances in new label strategies and application of liquid crystalline medium for giving additional structural information have improved the accuracy of solution protein structures determined by NMR, and extended the molecular weight limit. Several structures of proteins of 30 ku have been solved using 15N,13C,2H labeling multidimensional NMR techniques, and this limit will probably be surpassed in the near future. |
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| Keywords: | nuclear magnetic resonance protein three-dimensional structure liquid crystal dipolar coupling isotope labeling |
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