| cDNA芯片研发及其在肥胖患者脂肪基因差异表达研究中的应用 |
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| 引用本文： | 赵萸,骆天红,王侃侃,张济,郑培烝,赵春军,刘优萍,郑升,顾燕云,李果,罗敏.cDNA芯片研发及其在肥胖患者脂肪基因差异表达研究中的应用[J].生物化学与生物物理进展,2004,31(12):1067-1078. |
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| 作者姓名： | 赵萸 骆天红 王侃侃 张济 郑培烝 赵春军 刘优萍 郑升 顾燕云 李果 罗敏 |
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| 作者单位： | 1. 上海第二医科大学附属瑞金医院,上海市内分泌代谢病临床研究中心,上海市内分泌研究所,医学基因组学国家重点实验室,上海,200025
2. 上海第二医科大学附属瑞金医院,上海市血液病研究所,医学基因组学国家重点实验室,上海,200025 |
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| 基金项目： | 国家重点基础研究发展规划项目(973)（2002BA711A05）和国家自然科学基金资助项目(30100084，30100085). |
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| 摘 要： | 为了加快基因功能的研究，利用已有的来源于不同组织的cDNA克隆，并通过交换和购买补充了低丰度和染色体覆盖不完全的部分cDNA，研制开发出具有相当代表性、覆盖较完全的高密度cDNA表达型基因芯片，每张芯片上含有384个质控DNA和12 630个cDNA探针，其中包括12 508个Unigene和122个表达序列标签(EST).利用这些芯片，对肥胖患者及正常人内脏脂肪组织基因表达谱进行了初步研究，并发现在肥胖患者内脏脂肪组织差异表达的基因，其中上调的有与凋亡相关的基因、与免疫有关的基因以及与能量代谢有关的基因等，而下调的主要是与脂肪酸及胆固醇合成有关的基因，对这些基因进一步的功能研究将为阐明肥胖发生机制奠定基础.
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| 关 键 词： | cDNA芯片，肥胖，脂肪组织，差异表达 |
| 收稿时间： | 2004/6/16 0:00:00 |
| 修稿时间： | 2004/8/30 0:00:00 |
Development of High-density cDNA Microarray and Its Application in The Study of Differential Gene Expression Profile of Adipose Tissue From Obese Patients |
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| ZHAO Yu,LUO Tian-Hong,WANG Kan-Kan,ZHANG Ji,ZHENG Pei-Zheng,ZHAO Chun-Jun,LIU You-Ping,ZHENG Sheng,GU Yan-Yun,LI Guo and LUO Min.Development of High-density cDNA Microarray and Its Application in The Study of Differential Gene Expression Profile of Adipose Tissue From Obese Patients[J].Progress In Biochemistry and Biophysics,2004,31(12):1067-1078. |
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| Authors: | ZHAO Yu LUO Tian-Hong WANG Kan-Kan ZHANG Ji ZHENG Pei-Zheng ZHAO Chun-Jun LIU You-Ping ZHENG Sheng GU Yan-Yun LI Guo and LUO Min |
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| Institution: | Ruijin Hospital Attached to Shanghai Second Medical University, Shanghai Endocrine and Metabolic Diseases Clinical Research Center, Shanghai Institute of Endocrinology, State Key Laboratory of Medical Genomics, Shanghai 200025, China;Ruijin Hospital Attached to Shanghai Second Medical University, Shanghai Endocrine and Metabolic Diseases Clinical Research Center, Shanghai Institute of Endocrinology, State Key Laboratory of Medical Genomics, Shanghai 200025, China;Ruijin Hospital Attached to Shanghai Second Medical University, Shanghai Institute of Hematology, State Key Laboratory of Medical Genomics, Shanghai 200025, China;Ruijin Hospital Attached to Shanghai Second Medical University, Shanghai Institute of Hematology, State Key Laboratory of Medical Genomics, Shanghai 200025, China;Ruijin Hospital Attached to Shanghai Second Medical University, Shanghai Institute of Hematology, State Key Laboratory of Medical Genomics, Shanghai 200025, China;Ruijin Hospital Attached to Shanghai Second Medical University, Shanghai Institute of Hematology, State Key Laboratory of Medical Genomics, Shanghai 200025, China;Ruijin Hospital Attached to Shanghai Second Medical University, Shanghai Endocrine and Metabolic Diseases Clinical Research Center, Shanghai Institute of Endocrinology, State Key Laboratory of Medical Genomics, Shanghai 200025, China;Ruijin Hospital Attached to Shanghai Second Medical University, Shanghai Endocrine and Metabolic Diseases Clinical Research Center, Shanghai Institute of Endocrinology, State Key Laboratory of Medical Genomics, Shanghai 200025, China;Ruijin Hospital Attached to Shanghai Second Medical University, Shanghai Endocrine and Metabolic Diseases Clinical Research Center, Shanghai Institute of Endocrinology, State Key Laboratory of Medical Genomics, Shanghai 200025, China;Ruijin Hospital Attached to Shanghai Second Medical University, Shanghai Endocrine and Metabolic Diseases Clinical Research Center, Shanghai Institute of Endocrinology, State Key Laboratory of Medical Genomics, Shanghai 200025, China;Ruijin Hospital Attached to Shanghai Second Medical University, Shanghai Endocrine and Metabolic Diseases Clinical Research Center, Shanghai Institute of Endocrinology, State Key Laboratory of Medical Genomics, Shanghai 200025, China |
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| Abstract: | To accelerate study of gene function, a home-made high-density cDNA microarray was developed, using the cDNA clones from different types of tissues. In order to cover as far as possible the whole genome, cDNA clones that essentially represented genes with low abundance were introduced from Resgene Company. Totally 384 quality control DNA and 12 630 cDNA fragments, which includes 12 508 Unigenes and 122 ESTs, were spotted on the chip, after optimization. The home-made cDNA microarray was evaluated with Microarray ScoreCard and proved to be representative and reliable. These microarrays were then used to study the differential gene expression profiles of visceral adipose tissues between obese patients and normal subjects. The results showed that genes involved in immune-regulation, apoptosis and metabolism of energy were up-regulated in obese patients, while genes involved in lipid synthesis were down-regulated in obese patients. Further exploration of these genes will greatly improve the understanding of the pathogenesis of obesity. |
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| Keywords: | cDNA microarray obesity adipose tissue differential expression |
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