| 烟草果胶甲基酯酶（PME）基因的克隆及功能分析(英) |
| |
| 引用本文： | 李春波,高锋,钟永旺,魏春红,李毅.烟草果胶甲基酯酶（PME）基因的克隆及功能分析(英)[J].生物化学与生物物理进展,2004,31(7):643-649. |
| |
| 作者姓名： | 李春波 高锋 钟永旺 魏春红 李毅 |
| |
| 作者单位： | 北京大学生命科学学院,蛋白质工程和植物基因工程国家重点实验室,北大-耶鲁植物分子生物学及农业生物技术中心,北京,100871 |
| |
| 基金项目： | 国家杰出人才科学基金项目(30125004),国家重点基础研究发展规划项目（973）(G200000016201)和国家211工程资助项目. |
| |
| 摘 要： | 为了研究果胶甲基酯酶（pectin methyl-esterase，PME）（EC 3.1.1.11）与植物病毒运动蛋白之间的相互作用，应用RT-PCR方法从烟草(Nicotiana benthamiana)中克隆了PME基因，并测定了全序列(GenBank登录号AY238968).序列分析显示该基因由两个保守的结构域组成(PMEI和pectinesterase). DNA印迹结果表明,该基因在基因组中存在多个拷贝，蛋白质印迹表明,植物总蛋白中存在两种形式的PME蛋白，但RNA印迹结果显示,在烟草细胞中只检测到全长的PME转录产物.酵母双杂交结果表明,PME与水稻矮缩病毒Pns11(具有非特异的核酸结合活性)之间存在相互作用，而没有检测到PME与已知的运动蛋白Pns6之间的相互作用，推测Pns11蛋白可能参与了水稻矮缩病毒粒子的运动.
|
| 关 键 词： | 果胶甲基酯酶，序列分析，酵母双杂交，运动蛋白 |
| 收稿时间： | 2003/12/23 0:00:00 |
| 修稿时间： | 2004/1/10 0:00:00 |
Cloning and Functional Analysis of Tobacco Pectin Methylesterase |
| |
| LI Chun-Bo,GAO Feng,ZHONG Yong-Wang,WEI Chun-Hong and LI Yi.Cloning and Functional Analysis of Tobacco Pectin Methylesterase[J].Progress In Biochemistry and Biophysics,2004,31(7):643-649. |
| |
| Authors: | LI Chun-Bo GAO Feng ZHONG Yong-Wang WEI Chun-Hong and LI Yi |
| |
| Institution: | Peking-Yale Joint Center for Plant Molecular Genetics and Agrobiotechnology, The National Laboratory of Protein Engineering and Plant Genetic Engineering, College of Life Sciences, Peking University, Beijing 100871, China;Peking-Yale Joint Center for Plant Molecular Genetics and Agrobiotechnology, The National Laboratory of Protein Engineering and Plant Genetic Engineering, College of Life Sciences, Peking University, Beijing 100871, China;Peking-Yale Joint Center for Plant Molecular Genetics and Agrobiotechnology, The National Laboratory of Protein Engineering and Plant Genetic Engineering, College of Life Sciences, Peking University, Beijing 100871, China;Peking-Yale Joint Center for Plant Molecular Genetics and Agrobiotechnology, The National Laboratory of Protein Engineering and Plant Genetic Engineering, College of Life Sciences, Peking University, Beijing 100871, China;Peking-Yale Joint Center for Plant Molecular Genetics and Agrobiotechnology, The National Laboratory of Protein Engineering and Plant Genetic Engineering, College of Life Sciences, Peking University, Beijing 100871, China |
| |
| Abstract: | |
| |
| Keywords: | pectin methylesterase sequence analysis yeast two-hybrid system movement protein |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
| 点击此处可从《生物化学与生物物理进展》浏览原始摘要信息 |
| 点击此处可从《生物化学与生物物理进展》下载免费的PDF全文 |
|
