| 干扰RNA的非靶免疫副作用及其siRNA序列设计与化学修饰 |
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| 引用本文： | 韩秋菊,张彩,张建,田志刚.干扰RNA的非靶免疫副作用及其siRNA序列设计与化学修饰[J].生物化学与生物物理进展,2007,34(4):343-347. |
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| 作者姓名： | 韩秋菊 张彩 张建 田志刚 |
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| 作者单位： | 1. 山东大学药学院免疫药理与免疫治疗研究所,济南,250012
2. 山东大学药学院免疫药理与免疫治疗研究所,济南,250012;中国科学技术大学免疫学研究所,合肥,230027 |
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| 基金项目： | 国家重点基础研究发展计划(973计划) |
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| 摘 要： | siRNA介导的RNA干扰技术已经成为基因功能研究和开展疾病治疗的有用工具.近年发现,siRNA在哺乳动物体内可激活天然免疫系统,诱导干扰素等炎症因子的分泌,并且可非特异性抑制某些非靶基因的表达,有可能极大限制RNA干扰技术的应用.进行高效特异性siRNA的设计和修饰,以保持或者增强siRNA的特异性靶基因沉默作用,又消除siRNA对机体的非靶免疫副作用,成为使siRNA安全有效应用于临床治疗的关键.
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| 关 键 词： | RNA干扰 非靶效应 |
| 收稿时间： | 2/5/2007 12:00:00 AM |
| 修稿时间： | 2007-02-05 |
Off-target Effects of RNAi and Design of Highly Effective Modified siRNAs |
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| HAN Qiu-Ju,ZHANG Cai,ZHANG Jian and TIAN Zhi-Gang.Off-target Effects of RNAi and Design of Highly Effective Modified siRNAs[J].Progress In Biochemistry and Biophysics,2007,34(4):343-347. |
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| Authors: | HAN Qiu-Ju ZHANG Cai ZHANG Jian and TIAN Zhi-Gang |
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| Institution: | Institute of Immunopharmacology and Immunotherapy, Shandong University, Jinan 250012, China;Institute of Immunopharmacology and Immunotherapy, Shandong University, Jinan 250012, China;Institute of Immunopharmacology and Immunotherapy, Shandong University, Jinan 250012, China;Institute of Immunopharmacology and Immunotherapy, Shandong University, Jinan 250012, China;Institute of Immunology, University of Science and Technology of China, Hefei 230027, China |
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| Abstract: | RNA interference mediated by short interfering RNA is widely used to study functional genes and also being developed for therapeutic applications. However, recent study demonstrated that siRNA might activate innate immune system and induce huge production of inflammatory cytokines in mammals, and also randomly inhibit expression of undesired genes. Designing highly effective siRNAs or modifying the siRNA to retain or enhance the silence efficiency and meanwhile abolish the off-target effects associated with immunostimulation then become the key techniques in application of siRNAs as safe and effective therapeutic agents. |
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| Keywords: | siRNA TLR |
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