| 人及小鼠钠通道SCN3A基因的启动子及其上游调控区的分析 |
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| 引用本文： | 龙跃生,赵绮华,曾涛,曾杨,孙卫文,廖卫平.人及小鼠钠通道SCN3A基因的启动子及其上游调控区的分析[J].生物化学与生物物理进展,2009,36(3):339-345. |
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| 作者姓名： | 龙跃生 赵绮华 曾涛 曾杨 孙卫文 廖卫平 |
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| 作者单位： | 广州医学院第二附属医院神经科学研究所，神经遗传与离子通道病省部共建教育部重点实验，广州 510260;广州医学院第二附属医院神经科学研究所，神经遗传与离子通道病省部共建教育部重点实验，广州 510260;广州医学院第二附属医院神经科学研究所，神经遗传与离子通道病省部共建教育部重点实验，广州 510260;广州医学院第二附属医院神经科学研究所，神经遗传与离子通道病省部共建教育部重点实验，广州 510260;广州医学院第二附属医院神经科学研究所，神经遗传与离子通道病省部共建教育部重点实验，广州 510260;广州医学院第二附属医院神经科学研究所，神经遗传与离子通道病省部共建教育部重点实验，广州 510260 |
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| 基金项目： | 国家自然科学基金资助项目(30600198), 广东省自然科学基金博士科研启动项目(06301101)和广东省名医工程研究项目(粤卫[2004]18号). |
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| 摘 要： | 为了比较研究人与小鼠SCN3A基因的启动子及其上游调控区的特征，采用5′-Full RACE方法对人及小鼠SCN3A基因的转录起始点进行了准确定位，通过序列测定及对比分析证明：确定人和小鼠SCN3A基因的转录起始点均为“A”，人SCN3A基因转录起始点位于翻译起始点上游约27 kb处，而小鼠位于翻译起始点上游约31 kb处．人SCN3A基因5′非翻译区存在两个5′非翻译外显子，而小鼠只有一个5′非翻译外显子．人和小鼠SCN3A基因核心启动子区(-80 ～+70)的同源率高达96.0%，存在相同的启动子核心元件，BRE/和TATA；在-400至+200区段内预测到人存在而小鼠不存在的转录因子有PHR1、GATA-1、FOXN2、NF-1及AP-4，小鼠存在而人不存在的转录因子Sp、Sp3及GBF．人和小鼠SCN3A启动子区特征的异同将为进一步研究该基因在人和小鼠的表达调控机制提供重要线索．
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| 关 键 词： | 转录起始点，电压门控型钠通道，SCN3A，启动子 |
| 收稿时间： | 2008/6/23 0:00:00 |
| 修稿时间： | 8/6/2008 12:00:00 AM |
Characterization of The Promoter Region and Upstream Regulation Region of Human and Mouse SCN3A Gene |
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| LONG Yue-Sheng,ZHAO Qi-Hu,ZENG Tao,ZENG Yang,SUN Wei-Wen and LIAO Wei-Ping.Characterization of The Promoter Region and Upstream Regulation Region of Human and Mouse SCN3A Gene[J].Progress In Biochemistry and Biophysics,2009,36(3):339-345. |
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| Authors: | LONG Yue-Sheng ZHAO Qi-Hu ZENG Tao ZENG Yang SUN Wei-Wen and LIAO Wei-Ping |
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| Institution: | Institute of Neuroscience and The Second Affiliated Hospital of Guangzhou Medical College, Key Laboratory of Neurogenetics and Channelopathies of Guangdong Province and The Ministry of Education of China, Guangzhou 510260, China;Institute of Neuroscience and The Second Affiliated Hospital of Guangzhou Medical College, Key Laboratory of Neurogenetics and Channelopathies of Guangdong Province and The Ministry of Education of China, Guangzhou 510260, China;Institute of Neuroscience and The Second Affiliated Hospital of Guangzhou Medical College, Key Laboratory of Neurogenetics and Channelopathies of Guangdong Province and The Ministry of Education of China, Guangzhou 510260, China;Institute of Neuroscience and The Second Affiliated Hospital of Guangzhou Medical College, Key Laboratory of Neurogenetics and Channelopathies of Guangdong Province and The Ministry of Education of China, Guangzhou 510260, China;Institute of Neuroscience and The Second Affiliated Hospital of Guangzhou Medical College, Key Laboratory of Neurogenetics and Channelopathies of Guangdong Province and The Ministry of Education of China, Guangzhou 510260, China;Institute of Neuroscience and The Second Affiliated Hospital of Guangzhou Medical College, Key Laboratory of Neurogenetics and Channelopathies of Guangdong Province and The Ministry of Education of China, Guangzhou 510260, China |
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