A NEWLY ANTI-Streptococcus suis BACTERIOCIN PRODUCING STRAIN FROM UNWEANED PIGLETS FECAL MATTER: ISOLATION,PRELIMINARY IDENTIFICATION,AND OPTIMIZATION OF MEDIUM COMPOSITION FOR ENHANCED BACTERIOCIN PRODUCTION |
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| Authors: | Xiangmei Zhang Xiaoyuan Chang Guorong Liu Pengpeng Wu |
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| Institution: | 1. College of Food Science and Nutritional Engineering, China Agricultural University , Beijing , China;2. College of Biological Science and Engineering, Hebei University of Economics &3. Business , Shijiazhuang , China;4. College of Food Science and Nutritional Engineering, China Agricultural University , Beijing , China |
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| Abstract: | A newly isolated anti-Streptococcus suis bacteriocin-producing strain LPL1-5 was obtained from healthy unweaned piglets' fecal matter, and was designated as Lactobacillus pentosus LPL1-5 based on morphology, biochemical properties, and 16S rDNA sequencing analysis. The medium composition for enhanced bacteriocin production by L. pentosus LPL1-5 was optimized by statistical methodology. Yeast extract, K2HPO4 · 3H2O, and MnSO4 · H2O were identified as significant components influencing pentocin LPL1-5 production using the Plackett–Burman method. Response surface methodology was applied for further optimization. The concentrations of medium components for enhanced pentocin LPL1-5 production were as follows (g/L): lactose 20.00, tryptone 10.00, beef extract 10.00, yeast extract 14.00, MnSO4 · H2O 0.84, K2HPO4 · 3H2O 4.92, triammonium citrate 2.00, Na-acetate 5.00, MgSO4 · 7H2O 0.58, Tween 80 1.00. Under the optimized condition, a value of 3154.65 ± 27.93 IU/mL bacteriocin activity was achieved, which was 4.2-fold that of the original medium. |
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| Keywords: | bacteriocin isolation Lactobacillus pentosus optimization response surface methodology Streptococcus suis |
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