Preparative Isolation and Purification of Hydroxyanthraquinones from Rheum tanguticum Maxim. on Normal Phase Silica Gel: Using a Flash Master Personal System |
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| Authors: | Yong‐Zhi Zhang Dong‐Zhi Wei Gui‐Xin Chou En‐Yuan Zhu |
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| Institution: | 1. State Key Laboratory of Bioreactor Engineering , New World Institute of Biotechnology, East China University of Science and Technology , Shanghai, P. R. China;2. Institute of Chinese Materia Medica, Shanghai University of TCM , Shanghai, P. R. China |
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| Abstract: | Abstract A flash chromatography method for preparative separation and purification of five hydroxyanthraquinones from the Chinese medicinal herb Rheum tanguticum Maxim. ex Balf. was developed by using Flash Master Personal+ systems. The purities of the products, chrysophanol, physcion, emodin, aloe‐emodin, and rhein were all over 90%, determined by high performance liquid chromatography (HPLC). Their structures were ascertained by EI‐MS, 1H‐ and 13C‐NMR data, and by co‐TLC and HPLC with the authentic samples available in our laboratory. |
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| Keywords: | Hydroxyanthraquinones Flash chromatography Rheum tanguticum Maxim High performance liquid chromatography |
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