Impact of antigenic and genetic drift on the serologic surveillance of H5N2 avian influenza viruses |
| |
| Authors: | Magdalena Escorcia Karol Carrillo-Sánchez Santiago March-Mifsut Joaquin Chapa Eduardo Lucio Gerardo M Nava |
| |
| Institution: | 1.Departamento de Producción Animal Aves. Facultad de Medicina Veterinaria y Zootecnia,Universidad Nacional Autónoma de México. Ciudad Universitaria,Coyoacán, D. F.,México;2.Instituto Nacional de Medicina Genómica,Periférico Sur No. 4124, Torre Zafiro II, Piso 6. Col. Ex Rancho de Anzaldo,Alvaro Obregón. D. F,México;3.Investigación Aplicada S. A. de C. V. 7 Norte 416,Tehuacán, Puebla,México;4.Washington University School of Medicine. Dept,Pathology and Immunology,St. Louis,USA |
| |
| Abstract: | Background Serologic surveillance of Avian Influenza (AI) viruses is carried out by the hemagglutination inhibition (HI) test using reference
reagents. This method is recommended by animal health organizations as a standard test to detect antigenic differences (subtypes)
between circulating influenza virus, vaccine- and/or reference- strains. However, significant discrepancies between reference
antisera and field isolates have been observed during serosurveillance of influenza A viruses in pig and poultry farms. The
objective of this study was to examine the effects of influenza virus genetic and antigenic drift on serologic testing using
standard HI assays and reference reagents. Low pathogenic AI H5N2 viruses isolated in Mexico between 1994 and 2008 were used
for phylogenetic analysis of AI hemagglutinin genes and for serologic testing using antisera produced with year-specific AI
virus isolates. |
| |
| Keywords: | |
| 本文献已被 SpringerLink 等数据库收录！ |
|
