Transgenic Rice Expressing Ictb and
FBP/Sbpase Derived from Cyanobacteria Exhibits Enhanced
Photosynthesis and Mesophyll Conductance to CO2
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Authors: | Han Yu Gong Yang Li Gen Fang Dao Heng Hu Wen Bin Jin Zhao Hai Wang Yang Sheng Li |
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Institution: | 1 State Key Laboratory for Hybrid Rice, College of Life Sciences, Wuhan
University, Wuhan, China, ; 2 Engineering Research Centre for the Protection and Utilization of
Bioresource in Ethnic Area of Southern China, South-Central University for
Nationalities, Wuhan, China, ; Institute of Genetics and Developmental Biology, Chinese
Academy of Sciences, CHINA, |
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Abstract: | To find a way to promote the rate of carbon flux and further improve the
photosynthetic rate in rice, two CO2-transporting and fixing relevant
genes, Ictb and FBP/Sbpase, which were derived
from cyanobacteria with the 35SCaMV promotor in the respective constructs, were
transformed into rice. Three homologous transgenic groups with
Ictb, FBP/Sbpase and the two genes
combined were constructed in parallel, and the functional effects of these two
genes were investigated by physiological, biochemical and leaf anatomy analyses.
The results indicated that the mesophyll conductance and net photosynthetic rate
were higher at approximately 10.5–36.8% and 13.5–34.6%,
respectively, in the three groups but without any changes in leaf anatomy
structure compared with wild type. Other physiological and biochemical
parameters increased with the same trend in the three groups, which showed that
the effect of FBP/SBPase on improving photosynthetic capacity was better than
that of ICTB and that there was an additive effect in ICTB+FBP/SBPase. ICTB
localized in the cytoplasm, whereas FBP/SBPase was successfully transported to
the chloroplast. The two genes might show a synergistic interaction to promote
carbon flow and the assimilation rate as a whole. The multigene transformation
engineering and its potential utility for improving the photosynthetic capacity
and yield in rice were discussed. |
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