Diversity of Both the Cultivable Protease-Producing Bacteria and Bacterial Extracellular Proteases in the Coastal Sediments of King George Island,Antarctica |
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| Authors: | Ming-Yang Zhou Guang-Long Wang Dan Li Dian-Li Zhao Qi-Long Qin Xiu-Lan Chen Bo Chen Bai-Cheng Zhou Xi-Ying Zhang Yu-Zhong Zhang |
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| Institution: | 1. State Key Laboratory of Microbial Technology, Shandong University, Jinan, China.; 2. Marine Biotechnology Research Center, Shandong University, Jinan, China.; 3. SOA Key Laboratory for Polar Science, Polar Research Institute of China, Shanghai, P. R. China.; U.S. Geological Survey, United States of America, |
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| Abstract: | Protease-producing bacteria play a vital role in degrading sedimentary organic nitrogen. However, the diversity of these bacteria and their extracellular proteases in most regions remain unknown. In this paper, the diversity of the cultivable protease-producing bacteria and of bacterial extracellular proteases in the sediments of Maxwell Bay, King George Island, Antarctica was investigated. The cultivable protease-producing bacteria reached 105 cells/g in all 8 sediment samples. The cultivated protease-producing bacteria were mainly affiliated with the phyla Actinobacteria, Firmicutes, Bacteroidetes, and Proteobacteria, and the predominant genera were Bacillus (22.9%), Flavobacterium (21.0%) and Lacinutrix (16.2%). Among these strains, Pseudoalteromonas and Flavobacteria showed relatively high protease production. Inhibitor analysis showed that nearly all the extracellular proteases from the bacteria were serine proteases or metalloproteases. These results begin to address the diversity of protease-producing bacteria and bacterial extracellular proteases in the sediments of the Antarctic Sea. |
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