Activity Prediction and Molecular Mechanism of Bovine Blood Derived Angiotensin I-Converting Enzyme Inhibitory Peptides |
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| Authors: | Ting Zhang Shaoping Nie Boqun Liu Yiding Yu Yan Zhang Jingbo Liu |
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| Institution: | 1. Laboratory of Nutrition and Functional Food, Jilin University, Changchun, Jilin, China.; 2. State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang, Jiangxi, China.; Escola Paulista de Medicina, BRAZIL, |
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| Abstract: | Development of angiotensin I-converting enzyme (ACE, EC 3.4.15.1) inhibitory peptides from food protein is under extensive research as alternative for the prevention of hypertension. However, it is difficult to identify peptides released from food sources. To accelerate the progress of peptide identification, a three layer back propagation neural network model was established to predict the ACE-inhibitory activity of pentapeptides derived from bovine hemoglobin by simulated enzyme digestion. The pentapeptide WTQRF has the best predicted value with experimental IC50 23.93 μM. The potential molecular mechanism of the WTQRF / ACE interaction was investigated by flexible docking. |
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