Characterization of an airborne microbial community: A case study in the archive of the University of Coimbra,Portugal |
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| Institution: | 1. Department of Biomedical, Biotechnological and Translational Sciences, University of Parma, Italy;2. Department of Industrial Engineering, University of Florence, Italy;3. Central Institute of Graphic Arts, Ministry of Cultural Heritage and Activities and Tourism, Rome, Italy;4. Department of Industrial Engineering, University of Catania, Italy;5. Hygiene Unit, University Hospital of Parma, Italy;6. STEBICEF Department, Laboratory of Biology and Biotechnology for Cultural Heritage, University of Palermo, Italy;7. Department of Environmental Biology, “Sapienza” University of Rome, Italy;8. Department of Clinical and Experimental Medicine, University of Parma, Italy |
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| Abstract: | Understanding the structure of indoor airborne microbial communities could be useful in optimizing conservation and disinfection procedures in archive repositories, preventing the biodeterioration of stored collections. In this study we characterized the microbial air community inside the Archive of the University of Coimbra, by identifying different fungal and bacterial organisms retrieved from air samples. The microbial contamination was determined using conventional culture methods, and the isolates were typified using morphological techniques. Results indicated a low microbial air contamination (107 ± 12 CFU/m3), particularly regarding fungal propagules (6 ± 1 CFU/m3). Fungal isolates were identified using ITS-DNA sequencing. Among fungal isolates, Penicillium was the most frequent genus, and Penicillium griseofulvum was the predominant species. Simpson diversity index (1-D) was applied to phenotypic and genotypic results. Total phenotypic diversity varied from 0.4 to 0.8 and regarding fungal species, the diversity was higher than 0.5. These results were compared with previous analyses of the Archive's air, suggesting that short-term changes in atmospheric conditions may influence the indoor air microbial community structure. |
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