Biodegradation of organochlorine pesticides by bacteria grown in microniches of the porous structure of green bean coffee |
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| Institution: | 1. Department of Environmental Systems Engineering, National School of Biological Sciences, National Polytechnic Institute, Carpio y Plan de Ayala 11340 DF, México;2. Department of Biotechnology and Bioengineering, CINVESTAV, Av. Instituto Politécnico Nacional 2508 AP 14-740, Col. Zacatenco CP 07360, México;3. Department of Chemical Engineering, University of Salamanca, Plaza de la Merced s/n, 37008 Salamanca, Spain;1. Key Laboratory of Agri-food Safety of Anhui Province, Lab of Quality & Safety and Risk Assessment for Agro-products on Storage and Preservation (Hefei), Ministry of Agriculture, School of Resource and Environment, Anhui Agricultural University, Hefei 230036, China;2. School of Life Science, Anhui Agricultural University, Hefei 230036, China;3. Department of Molecular Biosciences and Bioengineering, University of Hawaii at Manoa, 1955 East–West Road, Honolulu, HI 957822, USA;1. Department of Microbiology and Virology, School of Pharmacy with the Division of Laboratory Medicine, Medical University of Silesia, Jagiellońska 4, 41-200 Sosnowiec, Poland;2. Department of Biochemistry, Faculty of Biology and Environmental Protection, University of Silesia, Jagiellońska 28, 40-032 Katowice, Poland;3. Department of Microbiology, Faculty of Biology and Environmental Protection, University of Silesia, Jagiellońska 28, 40-032 Katowice, Poland;1. Key Laboratory of Agricultural Environment in Universities of Shandong, National Engineering Laboratory for Efficient Utilization of Soil and Fertilizer Resources, College of Resources and Environment, Shandong Agriculture University, Taian 271018, China;2. College of Marine Life Sciences, Ocean University of China, Qingdao 266109, China |
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| Abstract: | In this paper, the authors propose a model for DDT biodegradation by bacteria grown in microniches created in the porous structure of green bean coffee. Five bacteria isolated from coffee beans, identified as Pseudomonas aeruginosa, P. putida, Stenotrophomonas maltophilia, Flavimonas oryzihabitans, and Morganella morganii. P. aeruginosa and F. oryzihabitans, were selected for pesticide degradation. Bacteria were selected according to their ability to grow on mineral media amended with: (a) glucose (10 g l?1), (b) peptone (2 g l?1), and (c) ground coffee beans (2 g l?1). These three media were supplemented with 50 mg l?1 of 1,1,1-trichloro-2,2-bis (4-chlorophenyl) ethane (DDT) and endosulfan. GC/MS analysis demonstrated that the greatest DDT removal was obtained in the medium supplemented with coffee beans, where 1,1-dichloro-2,2′-bis (4-chlorophenyl)ethylene (DDE), 1-chloro-2,2-bis (4-chlorophenyl) ethane (DDMU) and 2,2′-bis (p-chlorophenyl)ethanol (DDOH) were detected. DDMU is a product of the reductive dechlorination of DDE, which in this system could be carried out under the anaerobic conditions in microniches present in the porous structure of the coffee bean. This was supported by scanning electron microscopy. Green bean coffee could be used as a nutrient source and as a support for bacterial growth in pesticide degradation. |
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