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A new Bauhinia monandra galactose-specific lectin purified in milligram quantities from secondary roots with antifungal and termiticidal activities |
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| Authors: | Jayra D SouzaMaria BR Silva Adriana CC ArgoloThiago H Napoleão Roberto A SáMaria TS Correia Patrícia MG PaivaMichele DC Silva Luana CBB Coelho |
| Institution: | a Departamento de Bioquímica, Centro de Ciências Biológicas, Universidade Federal de Pernambuco, Av. Moraes Rego, s/n, Cidade Universitária, Recife, 50670-420 Pernambuco, Brazil b Centro Acadêmico do Agreste, Universidade Federal de Pernambuco, Nova Caruaru, Caruaru, 55002-970 Pernambuco, Brazil c Departamento de Ciências Animais, Universidade Federal Rural do Semi-Árido, Mossoró, 59625-900 Rio Grande do Norte, Brazil |
| Abstract: | This work describes the purification in milligram quantities of a lectin from Bauhinia monandra secondary roots (BmoRoL) and its antifungal and termiticidal activities. The BmoRoL (6.2 mg) was isolated through ammonium sulfate fractionation and affinity chromatography on guar gel. Native lectin was resolved as a single band on polyacrylamide gel electrophoresis for basic proteins. Under denaturing and reducing conditions it appeared as a unique glycosylated polypeptide of 26 kDa. The highest agglutination activity of BmoRoL was found with glutaraldehyde-treated rabbit erythrocytes. BmoRoL showed antifungal activity against phytopathogenic species of Fusarium and was more active on Fusarium solani. The lectin also showed termiticidal activity on Nasutitermes corniger workers and soldiers with LC50 of 0.09 and 0.395 mg ml−1 for 12 days. In conclusion, BmoRoL is a new antifungal and termiticidal lectin that can be purified in milligram quantities and has potential biotechnological application for control of agricultural pests. |
| Keywords: | Bauhinia monandra Root lectin Antifungal activity Termiticidal activity |
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