Monitoring of microwave emission of HRP system during the enzyme functioning |
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Authors: | YuD Ivanov AF Kozlov КА Malsagova ТО Pleshakova SG Vesnin VYu Tatur ND Ivanova VS Ziborov |
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Institution: | 1. Institute of Biomedical Chemistry, ul. Pogodinskaya 10, Moscow 119121, Russia;2. RES Ltd, Moscow, Russia;3. Foundation of Perspective Technologies and Novations, Moscow, Russia;4. Skryabin Moscow State Academy of Veterinary Medicine and Biotechnology, Moscow, Russia;5. Joint Institute for High Temperatures of the Russian Academy of Sciences, Moscow, Russia |
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Abstract: | Monitoring of microwave emission from aqueous solution of horseradish peroxidase (HRP) in the process of the enzyme functioning was carried out. For the monitoring, a system containing HRP, luminol and Н2О2 was employed. Microwave emission measurements were carried out in the 3.4-4.2 GHz frequency range using the active and passive modes (active-mode and passive-mode measurements). In the active mode, excitation of the solution in the pulsed electromagnetic field was accomplished. In the passive mode, no excitation was induced. It appears that the passive-mode measurements taken in the course of the peroxidase reaction in the enzyme system have shown a 0.5 °С increase of the microwave signal. Upon the active-mode measurements, taken in the same reaction conditions, the forced excitation of the solution has also led to the increase (by 2 °С) of the level of the microwave signal – i.e. to its 4-fold enhancement compared to the signal obtained in passive-mode measurements. |
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Keywords: | Horseradish peroxidase Microwave emission Enzyme system |
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