Phylogenetic analysis of Leptospira strains of pathogenic serovars using 23S rDNA gene sequences. |
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| Authors: | F Sasaki R Kano Y Nakamura H Tsujimoto S Yamamoto A Hasegawa |
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| Institution: | Department of Pathobiology, Nihon University School of Veterinary Medicine. |
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| Abstract: | The 23S ribosomal DNAs were amplified from 11 strains of Leptospira interrogans sensu lato by polymerase chain reaction (PCR) and sequenced. The PCR products of about 290-bp DNA fragments indicated more than 97% sequence similarity to each other. The phylogenetic tree based on the 23S ribosomal DNAs obtained in this study revealed that 11 strains of L. interrogans examined composed a cluster distinct to that of L. weilii and L. borgpetersenii, confirming that these strains were similar to strain Moulton of L. interrogans serovar canicola in 23S rDNA sequence. |
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