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Formation of viable but non-culturable state (VBNC) of Aeromonas hydrophila and its virulence in goldfish, Carassius auratus |
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| Authors: | M Habibur Rahman Satoru Suzuki Kenji Kawai |
| Institution: | aFish Disease Laboratory, Department of Aquaculture, Kochi University, Monobe, Nankou, Kochi 783-8502, Japan;bFisheries Research Laboratory, Department of Zoology, Rajshahi University, Rajshabi 6205, Bangladesh;cCentre for Marine Environmental Studies, Ehime University, Matsuyama 790-8577, Japan |
| Abstract: | In this study we investigated the viable but non-culturable (VBNC) state of Aeromonas hydrophila and its virulence in goldfish. Aeromonas hydrophila cultured in a 0.35% NaCl solution at pH 7.5 and at 25 °C for 50 days showed the VBNC state. In the VBNC state we were unable to detect viable bacteria by the plate count method but we did find 104 cells/ml by the direct viable count microscopical method after staining with fluorescein diacetate and ethidium bromide. The virulence comparison in goldfish showed that bacteria cultured at 25 °C for 1 day in a 0.35% NaCl solution were more virulent than bacteria cultured for 28 days. VBNC bacteria showed lower virulence in goldfish compared to 28-day-cultured bacteria by intraperitoneal injection.The results from the study suggest that A. hydrophila can remain in the aquatic environment for prolonged periods in the VBNC state but those cells are not pathogenic to goldfish. |
| Keywords: | Aeromonas hydrophila viable but non-culturable infection goldfish |
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