Differential susceptibility to hydrogen sulfide-induced apoptosis between PHLDA1-overexpressing oral cancer cell lines and oral keratinocytes: Role of PHLDA1 as an apoptosis suppressor |
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Authors: | Takatoshi Murata Tsutomu Sato Takeshi Kamoda Hiromitsu Moriyama Yasuo Kumazawa Nobuhiro Hanada |
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Institution: | 1. Department of Translational Research, Tsurumi University School of Dental Medicine, Yokohama 230-8501, Japan;2. Department of Oral Health, School of Life Dentistry, Nippon Dental University, Tokyo 102-0071, Japan;3. Department of Dental Hygiene, Nippon Dental University College at Tokyo, Tokyo 102-0071, Japan;4. Laboratory of Molecular and Cellular Biology, Faculty of Agriculture, Tokyo University of Agriculture and Technology, Tokyo 183-8509, Japan;5. Department of Oral Maxillofacial Surgery, Nippon Dental University Hospital, Tokyo 102-8158, Japan |
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Abstract: | Hydrogen sulfide (H2S) is a novel gasotransmitter that plays multiple biological roles in various body systems. In addition to its endogenous production, H2S is produced by bacteria colonizing digestive organs, including the oral cavity. H2S was previously shown to enhance pro-apoptotic effects in cancer cell lines, although the mechanisms involved remain unclear. To properly assess the anti-cancer effects of H2S, however, investigations of apoptotic effects in normal cells are also necessary. The aims of this study were (1) to compare the susceptibility to H2S-induced apoptosis between the oral cancer cell line Ca9-22 and oral keratinocytes that were derived from healthy gingiva, and (2) to identify candidate genes involved in the induction of apoptosis by H2S. The susceptibility to H2S-induced apoptosis in Ca9-22 cells was significantly higher than that in keratinocytes. H2S exposure in Ca9-22 cells, but not keratinocytes, enhanced the expression of pleckstrin homology-like domain, family A, member 1 (PHLDA1), which was identified through a differential display method. In addition, PHLDA1 expression increased during actinomycin D-induced apoptosis in Ca9-22 cells. Knockdown of PHLDA1 expression by small interfering RNA in Ca9-22 cells led to expression of active caspase 3, thus indicating apoptosis induction. The tongue cancer cell line SCC-25, which expresses PHLDA1 at a high level, showed similar effects. Our data indicate that H2S is an anti-cancer compound that may contribute to the low incidence of oral cancer. Furthermore, we demonstrated the role of PHLDA1 as an apoptosis suppressor. |
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Keywords: | H2S hydrogen sulfide PHLDA1 pleckstrin homology-like domain family A member 1 ACP annealing control primers Annexin V-PE phycoerythrin-conjugated annexin V 7-AAD 7-amino-actinomycin D siRNA small interfering RNA ANOVA analysis of variance SEM standard error of the mean |
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