Alterations in the characteristics of 2-deoxy D-glucose transport into cultured human glioma cells during cell growth. |
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Authors: | E Walum A Edström |
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Institution: | Department of Zoophysiology, University of Göteborg, S-400 33 Göteborg, Sweden |
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Abstract: | The uptake of 2-deoxy-d-glucose (2-DG) into human glioma cells (138 MG) was related to cell growth. The uptake of 2-DG was high at confluency but low in both rapidly growing sparse cultures and in growth-inhibited dense cultures. Lineweaver-Burke plots of uptake at different cell densities showed changes in Vmax; Km, however, remained constant. Dibutyryl cyclic-AMP (db-cAMP) doubled the uptake of 2-DG into rapidly growing sparse cultures but lacked effect at higher cell density. Independent of their density, cells treated with db-cAMP attained the characteristic morphology of differentiated glial cells. |
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