Bioactivity-based analysis and chemical characterization of hypoglycemic and antioxidant components from Artemisia argyi |
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| Institution: | 1. School of Traditional Chinese Pharmacy, China Pharmaceutical University, 24 Tongjiaxiang, Nanjing 210009, China;2. School of Pharmacy, China Pharmaceutical University, 24 Tongjiaxiang, Nanjing 210009, China;3. Faculty of Pharmaceutical Sciences, Toho University, Miyama 2-2-1, Funabashi, Chiba 274-8510, Japan;4. Department of Medicinal Molecular Chemistry, Osaka University of Pharmaceutical Sciences, 4-20-1 Nasahara, Takatsuki, Osaka 569-1094, Japan;5. Jiangsu Food and Pharmaceutical Science College, Huaian, Jiangsu 223003, China;1. Academy of Integrative Medicine, Dalian Medical University, Dalian 116044, China;2. Department of Biotechnology, Dalian Medical University, Dalian 116044, China;3. Cutaneous Biology Research Center, Massachusetts General Hospital and Harvard Medical School, Charlestown, MA 02129, USA;1. Core Laboratory for Clinical Medical Research, Beijing Tian Tan Hospital, Capital Medical University, Beijing, China;2. Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, China;3. Department of Colorectal and Anal Surgery, The First Hospital of Jilin University, Changchun, China |
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| Abstract: | Diabetes is one of the metabolic disorders in the world. It is the prime reason of mortality and morbidity owing to hyperglycemia which is link with numerus obstacles. Artemisia argyi is commonly used as an ingredient in healthy foods as well as an herbal medicine in Asian countries. The present research aims to evaluate the hypoglycemic effects of A. argyi and reveal its the potentially active constituents. The chemical composition was identified by HPLC-DAD-Q-TOF-MS, and fractionation was performed by extraction. The fractions were assessed by the blood glucose level, oral glucose tolerance and small intestinal α-glucosidase inhibitory tests, and an analysis of the total phenolic content (TPC), antioxidant and α-glucosidase inhibitory activities. In our efforts to characterize the compounds responsible for hypoglycemic effect, bioactivity-guided fraction of the MeOH extract and chemical investigation of its active EtOAc fraction led to the successful identification of caffeoylquinic acids, which were elucidated by molecular docking, using the crystal structure of S. cerevisiae isomaltase (PD code: 3AXI). In summary, this bio-guided search revealed that caffeoylquinic acids from A. argyi as potential active constituents displayed with hypoglycemic activity, which provided a basis for further study of pharmacological activity. |
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| Keywords: | Hypoglycemic activity Antioxidant activity α-glucosidase inhibitory activity |
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