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In vivo CD4+ T-cell up-regulation and high dose side effects of refolded duck interleukin-2
Authors:Wang Jinyong  Qi Jing  Shen Huigang  Zhou Jiyong  Fang Jie  Chen Hongxun  Wang Zhiping  Li Hongliang
Institution:Laboratory of Virology and Immunology, Institute of Preventive Veterinary Medicine, Zhejiang University, Hangzhou 310029, China.
Abstract:The recombinant duck interleukin-2 (rduIL-2) monomer was firstly isolated under nature condition, and refolded by oxidization procedure. Refolded rduIL-2 monomer induced in vitro proliferation of Con A-stimulated duck splenocytes in a sensitive and dose-dependent manner, and up-regulated in vivo the amounts of CD4+ T cells with low dose of administration. However, high doses intermittent administration resulted in sever side effects in vivo, with typical lymphocytic interstitial pneumonitis and nephritis, and lymphocytic depletion in splenic corpuscle. Our findings might be beneficial to studies of both mechanism and applications in vivo of avian IL-2.
Keywords:Recombinant duck interleukin-2  Monomer  Refolding  Lymphocyte proliferation  Immunophenotypic T cell  Toxicity
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