Micro RNA-19a suppresses IL-10 in peripheral B cells from patients with atherosclerosis |
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| Institution: | 1. Department of Cardiac Surgery, Zhejiang Hospital, Lingyin Rd. 12, 310013 Hangzhou, China;2. Department of Cardiology, Danyang People''s Hospital, West Xinmin Rd. 5, 212300 Danyang, China;3. Department of Cardiothoracic and Operation Theater, Zhejiang Provincial People''s Hospital, Shangtang Rd. 158, 310014 Hangzhou, China;4. School of Medical Science and Laboratory Medicine, Jiangsu University, XueFu Rd. 301, Zhenjiang, China;5. Institute of Molecular Cardiology, Heinrich-Heine University of Düsseldorf, Moorenstr. 5, 40225 Düsseldorf, Germany;1. Washington University School of Medicine in St Louis, St Louis, Missouri;2. University of Oklahoma Health Sciences Center, Oklahoma City, Oklahoma;3. Pediatrix Cardiology, Springfield, Missouri;4. Saint Louis University School of Medicine, St Louis, Missouri;5. University of Missouri Kansas City School of Medicine, Kansas City, Missouri;6. University of Nebraska Medical Center, Omaha, Nebraska |
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| Abstract: | Background and aimsThe interleukin (IL)-10-production B cells play an important role in the pathogenesis of atherosclerosis (Asro) with unknown mechanism. Micro RNA (miR)-17-92 cluster has strong immune regulatory activities. This study tests a hypothesis that miR-17-92 cluster suppresses IL-10 expression in B cells of Asro patients.MethodsPatients with Asro were recruited into this study. Peripheral blood samples were collected from the patients. B cells were isolated from the blood samples and analyzed to elucidate the role of miR-17-92 in the regulation of IL-10 expression.ResultsPeripheral B cells from patients with Asro show lower levels of IL-10 than that from healthy subjects. The IL-10 expression in the B cells is negatively correlated with the expression of miR-19a in the B cells. The serum levels of tumor necrosis factor (TNF)-α, interferon (IFN)-γ and interleukin (IL)-4 in Asro patients were higher than healthy subjects. Exposure to TNF-α or IFN-γ or IL-4 suppressed IL-10 expression in B cells via increasing the expression of miR-19a in B cells, which could be abolished by Inhibition of miR-19a.ConclusionsTNF-α or IFN-γ or IL-4 suppresses IL-10 in B cells via up regulating miR-19a expression. |
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| Keywords: | Interleukin-10 Lymphocyte Atherosclerosis Micro RNA p300 |
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