A short course of infusion of a hydrogen sulfide-donor attenuates endotoxemia induced organ injury via stimulation of anti-inflammatory pathways,with no additional protection from prolonged infusion |
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Authors: | Hamid Aslami Charlotte JP Beurskens Friso M de Beer Maria T Kuipers Joris JTH Roelofs Maria A Hegeman Koen F Van der Sluijs Marcus J Schultz Nicole P Juffermans |
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Institution: | 1. Laboratory of Experimental Intensive Care and Anesthesiology (LEICA), Academic Medical Center, Amsterdam, The Netherlands;2. Department of Pathology, Academic Medical Center, Amsterdam, The Netherlands;3. Department of Intensive Care Medicine, Academic Medical Center, Amsterdam, The Netherlands |
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Abstract: | Organ failure is associated with increased mortality and morbidity in patients with systemic inflammatory response syndrome. Previously, we showed that a short course of infusion of a hydrogen sulfide (H2S) donor reduced metabolism with concurrent reduction of lung injury. Here, we hypothesize that prolonged H2S infusion is more protective than a short course in endotoxemia with organ failure. Also, as H2S has both pro- and anti-inflammatory effects, we explored the effect of H2S on interleukin production.Endotoxemia was induced by an intravenous bolus injection of LPS (7.5 mg/kg) in mechanically ventilated rats. H2S donor NaHS (2 mg/kg) or vehicle (saline) was infused and organ injury was determined after either 4 or 8 h. A short course of H2S infusion was associated with reduction of lung and kidney injury. Prolonged infusion did not enhance protection. Systemically, infusion of H2S increased both the pro-inflammatory response during endotoxemia, as demonstrated by increased TNF-α levels, as well as the anti-inflammatory response, as demonstrated by increased IL-10 levels. In LPS-stimulated whole blood of healthy volunteers, co-incubation with H2S had solely anti-inflammatory effects, resulting in decreased TNF-α levels and increased IL-10 levels. Co-incubation with a neutralizing IL-10 antibody partly abrogated the decrease in TNF-α levels. In conclusion, a short course of H2S infusion reduced organ injury during endotoxemia, at least in part via upregulation of IL-10. |
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