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Dy and Nd induced genetic mutation of bacillus α-amylase
Authors:Dongyan Zhang  Jianhua Hu  Tong Zhang
Institution:a School of Chemical Engineering, Department of Biological Engineering, Inner Mongolia University of Technology, Hohhot 010051, China
b Inner Mongolia Academy of Agricultural Science, Hohhot 010031, China
Abstract:After treatment with DyCl3 and NdCl3, two strains of mutated bacilli showing increased α-amylase activity were isolated. The α-amylase genes were amplified, cloned, and sequenced. Sequencing revealed that there were either 11 or 14 bases altered in the two genes. These alterations took the form of base substitutions, and transversion was more common than transition. Based on these results, it was concluded that the rare earth compounds DyCl3 and NdCl3 were mutagens.
Keywords:Rare-earth  amyE  Mutation
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