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Acute metal stress in Populus tremula×P. alba (717‐1B4 genotype): Leaf and cambial proteome changes induced by cadmium2+ |
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| Authors: | Thomas C Durand Kjell Sergeant Sébastien Planchon Sabine Carpin Philippe Label Domenico Morabito Jean‐François Hausman Jenny Renaut |
| Institution: | 1. CRP‐Gabriel Lippmann, Department Environment and Agro‐biotechnologies, Belvaux, GD, Luxembourg;2. UFR‐Faculté des Sciences, Laboratoire de Biologie des Ligneux et des Grandes Cultures, Université d'Orléans, Orléans, France;3. INRA, USC2030 ‘Arbres et Réponses aux Contraintes Hydrique et Environnementales’ (ARCHE), Orléans, France;4. Institut National de la Recherche Agronomique, Ardon, Orléans, France |
| Abstract: | The comprehension of metal homeostasis in plants requires the identification of molecular markers linked to stress tolerance. Proteomic changes in leaves and cambial zone of Populus tremula×P. alba (717‐1B4 genotype) were analyzed after 61 days of exposure to cadmium (Cd) 360 mg/kg soil dry weight in pot‐soil cultures. The treatment led to an acute Cd stress with a reduction of growth and photosynthesis. Cd stress induced changes in the display of 120 spots for leaf tissue and 153 spots for the cambial zone. It involved a reduced photosynthesis, resulting in a profound reorganisation of carbon and carbohydrate metabolisms in both tissues. Cambial cells underwent stress from the Cd actually present inside the tissue but also a deprivation of photosynthates caused by leaf stress. An important tissue specificity of the response was observed, according to the differences in cell structures and functions. |
| Keywords: | Cadmium Cambium Fluorescence two‐dimensional difference gel electrophoresis Metal stress Plant proteomics Phytoremediation |