First evidence of a membrane‐bound,tyramine and β‐phenylethylamine producing,tyrosine decarboxylase in Enterococcus faecalis: A two‐dimensional electrophoresis proteomic study |
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Authors: | Enrica Pessione Alessandro Pessione Cristina Lamberti Daniel Jean Coïsson Kathrin Riedel Roberto Mazzoli Silvia Bonetta Leo Eberl Carlo Giunta |
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Institution: | 1. Dipartimento di Biologia Animale e dell'Uomo, University of Torino, Torino, Italy;2. DISCAFF, University of Piemonte Orientale “A.Avogadro”, Novara, Italy;3. Institute of Plant Biology, Department of Microbiology, University of Zurich, Zurich, Switzerland;4. Dipartimento di Scienze dell'Ambiente e della Vita, University of Piemonte Orientale “A.Avogadro”, Alessandria, Italy |
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Abstract: | The soluble and membrane proteome of a tyramine producing Enterococcus faecalis, isolated from an Italian goat cheese, was investigated. A detailed analysis revealed that this strain also produces small amounts of β‐phenylethylamine. Kinetics of tyramine and β‐phenylethylamine accumulation, evaluated in tyrosine plus phenylalanine‐enriched cultures (stimulated condition), suggest that the same enzyme, the tyrosine decarboxylase (TDC), catalyzes both tyrosine and phenylalanine decarboxylation: tyrosine was recognized as the first substrate and completely converted into tyramine (100% yield) while phenylalanine was decarboxylated to β‐phenylethylamine (10% yield) only when tyrosine was completely depleted. The presence of an aspecific aromatic amino acid decarboxylase is a common feature in eukaryotes, but in bacteria only indirect evidences of a phenylalanine decarboxylating TDC have been presented so far. Comparative proteomic investigations, performed by 2‐DE and MALDI‐TOF/TOF MS, on bacteria grown in conditions stimulating tyramine and β‐phenylethylamine biosynthesis and in control conditions revealed 49 differentially expressed proteins. Except for aromatic amino acid biosynthetic enzymes, no significant down‐regulation of the central metabolic pathways was observed in stimulated conditions, suggesting that tyrosine decarboxylation does not compete with the other energy‐supplying routes. The most interesting finding is a membrane‐bound TDC highly over‐expressed during amine production. This is the first evidence of a true membrane‐bound TDC, longly suspected in bacteria on the basis of the gene sequence. |
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Keywords: | Biogenic amines Broad specificity enzymes Food safety Membrane proteome Pirydoxal‐phosphate‐dependent decarboxylases |
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