Genetic analysis of replicating instabilities in yeast |
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Authors: | A Nasim C Grant |
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Institution: | Biology and Health Physics Division, Atomic Energy of Canada Limited, Chalk River, Ontario, Canada |
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Abstract: | Strains showing ethyl methanesulfonate (EMS)-induced replicating instability were genetically analysed to test whether within a given line, mosaics from different plating generations carry a mutation at the same site within the locus. A forward mutation system involving five loci controlling adenine biosynthesis in Schizosaccharomyces pombe was used. Genetic analysis was carried out by interallelic complementation and intragenic recombination tests. The data showed that EMS-induced instabilities are site-specific in being confined to the same recombination unit. This finding is discussed in relation to the possible mechanism(s) of replicating instabilities after different mutagenic treatments in a variety of biological systems. |
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Keywords: | EMS ethyl methanesulfonate MNNG MEAM malt extract agar medium MMA minimal medium YEA yeast extract agar medium |
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