Tracking algorithms chase down pathogens |
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Authors: | Nicolas Chenouard Alexandre Dufour Jean-Christophe Olivo-Marin Dr |
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Institution: | Institut Pasteur, Unité d'Analyse d'Images Quantitative, CNRS URA 2582, Paris, France |
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Abstract: | Understanding subcellular dynamic processes governing pathogenic mechanisms is a necessary step towards the development of new drugs and strategies against infectious diseases. Subcellular pathogenic mechanisms, such as viral invasion processes involve highly dynamic nanometric-scale objects and rapid molecular interactions that require the study of individual particle paths. Single-particle tracking methods allow visualizing and characterizing the dynamics of biological objects, and provide a straightforward and accurate means to understand subcellular processes. This review describes a number of particle-tracking methods in time-lapse microscopy sequences and provides examples of using such techniques to investigate mechanisms of host-pathogen interactions. |
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Keywords: | Cell tracking Motility Particle tracking Pathogens Shape |
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