A small interfering RNA targeting coxsackievirus B3 protects permissive HeLa cells from viral challenge |
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Authors: | Ahn Jeonghyun Jun Eun Seok Lee Hui Sun Yoon Seung Yong Kim DongHou Joo Chul-Hyun Kim Yoo Kyum Lee Heuiran |
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Institution: | Department of Microbiology, University of Ulsan College of Medicine, Seoul, Korea. |
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Abstract: | We examined the ability of small interfering RNAs (siRNAs) to disrupt infection by coxsackievirus B3 (CVB3). The incorporation of siRNAs dramatically decreased cell death in permissive HeLa cells in parallel with a reduction in viral replication. Three of four siRNAs had potent anti-CVB3 activity. The present study thus demonstrates that the antiviral effect is due to the downregulation of viral replication. In addition, an effective CVB3-specific siRNA had similar antiviral effects in other related enteroviruses possessing sequence homology in the targeted region. Because the CVB3-specific siRNA is effective against other enteroviruses, siRNAs have potential for a universal antienterovirus strategy. |
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