Application of an enzyme immunoassay for detecting antibodies in sera of Macaca fascicularis naturally exposed to Mycobacterium tuberculosis. |
| |
Authors: | K D Corcoran C O Thoen |
| |
Institution: | United States Army Medical Research Institute of Chemical Defense, Veterinary Medicine and Laboratory Resources Division, Aberdeen Proving Ground, MD 21010-5425. |
| |
Abstract: | An enzyme immunoassay (EIA) was developed for detecting mycobacterial antibodies in the sera of 22 Macaca fascicularis following a natural outbreak of tuberculosis. EIAs were conducted using four antigens (lysozyme, triton, or deoxycholate extracts of Mycobacterium tuberculosis or a purified protein derivative) and two conjugates (protein A or antihuman). Mycobacterial antibodies were detected in two of two culture-positive monkeys, in nine of ten tuberculin test-suspect monkeys (culture-negative), and in five of ten tuberculin test-negative monkeys (culture-negative). Results indicate EIA may be of practical value in detecting monkeys exposed to M. tuberculosis. |
| |
Keywords: | tuberculosis nonhuman primates enzyme immunoassay |
|
|