亚洲玉米螟不同地理种群基于COⅠ基因序列初步分析

本研究通过对我国亚洲玉米螟Ostrinia furnacalis(Guenée)6个地理种群的线粒体DNACOⅠ基因进行测序,运用分子进化遗传分析软件MEGA5对序列进行对比和同源性分析,并建立系统发育关系。结果表明,COⅠ基因在6个地理种群中进化具有一定的差异,平均遗传距离为0.013;进化树显示,亚洲玉米螟的三亚种群、广州种群与南昌种群聚为一支,然后与泰安种群聚为一支,再与哈尔滨种群聚为一支;而廊坊种群另聚成一支。遗传分歧度与系统发育进化关系均表明不同地理种群的遗传分化与地理距离之间具有一定的相关性。

Variation of mtDNA CO I sequences in different geographic populations of the Asian corn borer, Ostrinia furnacalis

The mitochondrial DNA （mtDNA） cytochrome oxidase subunlt I （COI） of 6 geo-populations of Ostrinia furnacalis （Guenee） was sequenced. The identity and variation of the resultant sequences and their phylogenic relationships were established by Mega 5. The results show that there are some differences in CO I sequences among different geo-populations of O. furnacalis and that the average evolutionary divergence between populations is 0. 012. Molecular phylogeny showed that the Sanyan, Guangzhou and Nanchang populations formed one branch, and the Taian, Haerbin and Langfang populations formed separate branches. The results of evolutionary divergence and molecular phylogeny suggest that the separation of different geo-populations of O. furnacalis is, at least to some extent, relative to geographic distance.