| 鉴定转Bt甘蔗抗条螟的方法 |
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| 引用本文： | 劳方业,翁丽星,李奇伟,陈勇生,汪联辉,陈健文,陈仲华,张琼,沈万宽,陈海宁,邓海华.鉴定转Bt甘蔗抗条螟的方法[J].昆虫知识,2011,48(4):1098-1102. |
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| 作者姓名： | 劳方业 翁丽星 李奇伟 陈勇生 汪联辉 陈健文 陈仲华 张琼 沈万宽 陈海宁 邓海华 |
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| 作者单位： | 1. 广州甘蔗糖业研究所,广东省甘蔗改良与生物炼制重点实验室,广州,510316
2. 复旦大学生命科学学院微生物学和微生物工程系,上海,200433 |
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| 基金项目： | 广东省教育部产学研结合项目，广东省科技计划项目，现代农业产业技术体系建设专项资金资助 |
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| 摘 要： | 通过采集蔗田条螟Proceras venosatus Walker的蛹,在室内可控条件下进行羽化、交配、产卵、孵化的方式获得虫源,并将初孵幼虫接于检疫温室桶栽的13个转Bt基因甘蔗品系的心叶上,7 d后调查幼虫的生长死亡情况以及甘蔗受害程度.结果表明,综合幼虫的生长死亡情况和甘蔗受害程度二项指标,能更准确评价甘蔗的抗虫...
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| 关 键 词： | 转Bt甘蔗 条螟 抗虫性 鉴定方法 |
Method to identify insect resistance of Bt-transgenic sugarcane lines |
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| LAO Fang-Ye,WENG Li-Xing,LI Qi-Wei,CHEN Yong-Sheng,WANG Lian-Hui,CHEN Jian-Wen,CHEN Zhong-Hua,ZHANG Qiong,SHEN Wan-Kuan,CHEN Hai-Ning,DENG Hai-Hua.Method to identify insect resistance of Bt-transgenic sugarcane lines[J].Entomological Knowledge,2011,48(4):1098-1102. |
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| Authors: | LAO Fang-Ye WENG Li-Xing LI Qi-Wei CHEN Yong-Sheng WANG Lian-Hui CHEN Jian-Wen CHEN Zhong-Hua ZHANG Qiong SHEN Wan-Kuan CHEN Hai-Ning DENG Hai-Hua |
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| Institution: | LAO Fang-Ye1 WENG Li-Xing2 LI Qi-Wei1 CHEN Yong-Sheng1 WANG Lian-Hui2 CHEN Jian-Wen1 CHEN Zhong-Hua1 ZHANG Qiong1 SHEN Wan-Kuan1 CHEN Hai-Ning1 DENG Hai-Hua1 (1.Guangzhou Sugarcane Industry Research Institute,Guangdong Key Lab of Sugarcane Improvement and Biorefinery,Guangzhou 510316,China,2.Department of Microbiology and Microbial Engineering,School of Life Sciences,Fudan University,Shanghai 200433,China) |
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| Abstract: | The relative resistance of 13 Bt-transgenic sugarcane lines to insect pests was investigated using plants planted in buckets in a quarantine greenhouse.Stem borer neonates Proceras venosatus Walker were obtained by collecting sugarcane borer pupae from cane fields and allowing these to undergo eclosion,mate,oviposit and hatch under controlled,indoor conditions.Newly hatched larvae were then inoculated into the leaf spindle of sugarcane plants.The growth and survival of larvae and the degree of plant damage ... |
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| Keywords: | Bt-transgenic sugarcane Proceras venosatus insect resistance method for identification |
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