黄杜鹃提取物对亚洲玉米螟生物活性的研究

本文报道了闹羊花素 - ( Rhodojaponin- ,简称 R- )和黄杜鹃花乙酸乙酯 ( Et OAc)提取物对亚洲玉米螟 Ostrenia furnacalis Guenée3龄幼虫的拒食、生长抑制和毒杀作用。以饲料柱法测定 R- 和 Et OAc提取物的非选择性拒食作用 AFC50 为 77.0 2和 384 .30 mg/ kg,而以虫重法表示时 ,则分别为15.60和 2 55.4 4 mg/ kg。以 R- 2 0 0 mg/ kg连续饲喂 4天后 ,换为无毒饲料至 15天 ,试虫死亡率为86.67% ,L T50 为 2 .99天 ;以 Et OAc提取物 2 0 0 0 mg/ kg处理时 ,同期的死亡率 66.67% ,LT50 为 3.85天。R- 和 Et OAc提取物的生长抑制中浓度 IC50 分别为 9.30和 39.64mg/ kg。文中还讨论了亚洲玉米螟拒食作用的测定方法和 R- “取食后效应”等作用特点

Impacts of bioactivitses of extracts from yellow azalea, Rhododendron molle G.Don on larvae development of Ostrinia furnacalis.

Antifeeding, poisoning and growth inhibition properties of Rhodojaponin- Ⅲ (Abbr. R- Ⅲ) and ethyl acetate (EtOAc) extracts of flowers from yellow azalez,Rhododendron molle G. Don on the 3rd instar larvae of Ostrinia furnacalis Guenée were reported. The values of AFCs0 (median antifeeding concentration) of R- Ⅲ and EtOAc extract were 77.02 and 384.30 mg/kg diet with column-shaped diet method, but were 15.60 and 255.44 mg/kg diet with body weight method, respectively. After 4d treatment with 2000 mg/kg R- Ⅲ and 200 mg/kg EtOAc extract, 86.67% and 66.67% mortality were obtained, as well as 2.99 and 3.85d for LTs0(lethal median time) on 15th day after treatment respectively. After 15d treatment with R-Ⅲ and EtOAc extracts the vgalues of ICs0 (medium inhibitory growth concentration)were 9.30 and 39.64 mg/kg diet, respectively. The measure method and action characters of R- Ⅲ and EtOAc extracts such as postingestive effects, were also discussed.