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| 刺吸电波图实验中金丝与烟粉虱的粘连技术 | |
| 引用本文： | 岳梅,罗晨,徐洪富,高庆刚,张芝利.刺吸电波图实验中金丝与烟粉虱的粘连技术[J].昆虫知识,2005,42(3):326-328. |
| 作者姓名： | 岳梅 罗晨 徐洪富 高庆刚 张芝利 |
| 作者单位： | 1. 山东农业大学植保学院昆虫系,泰安,271018;北京市农林科学院植保环保所,北京,100089 2. 北京市农林科学院植保环保所,北京,100089 3. 山东农业大学植保学院昆虫系,泰安,271018 |
| 基金项目： | 北京市科技新星项目(H02081350130)，北京市自然科学基金(6022007)。 |
| 摘 要： | 刺吸电波图(EPG)是用于研究刺吸式口器昆虫取食的电生理技术。实验中,昆虫粘连技术是一项非常关键的技术,直接影响实验结果。该文以烟粉虱Bemisiatabaci(Gennadius) ,为例,介绍了粉虱成虫和若虫粘连的具体步骤和注意细节,比较了不同粘连方法的优缺点。结果表明,冷冻法是粘连粉虱最好的一种方法。 |
| 关 键 词： | 刺吸电图 烟粉虱 粘连技术 |
Gluing techniques of gold wire electrode to Bemisia tabaci in electrical penetration graph |
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| Yue Mei,LUO Chen,Xu Hong-fu,GAO Qing-Gang,ZHANG Zhi-Li.Gluing techniques of gold wire electrode to Bemisia tabaci in electrical penetration graph[J].Entomological Knowledge,2005,42(3):326-328. | |
| Authors: | Yue Mei LUO Chen Xu Hong-fu GAO Qing-Gang ZHANG Zhi-Li |
| Institution: | YUE Mei~1,2,LUO Chen~2 **,XU Hong-Fu1,GAO Qing-Gang~1,2,ZHANG Zhi-Li1 |
| Abstract: | EPG is an electrical physiological technology which is used to study the probing and feeding behavior of piercing sucking insects. The insect gluing procedure is the pivotal skill which can affect the experiment result directly. In this article, we take Bemisia tabaci(Gennadius) as an example to introduce the detailed steps and gluing skills for the nymph and adult whitefly study. We also compare the different insect-handling methods. We suggest that the cold plate method is the best and convenient to glue whiteflies. |
| Keywords: | EPG Bemisia tabaci(Gennadius) gluing technique |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |