| 蓟马基因组DNA提取方法的改进 |
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| 引用本文： | 张利娟,沈登荣,张宏瑞,张宏伟,李正跃.蓟马基因组DNA提取方法的改进[J].昆虫知识,2011,48(3):775-781. |
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| 作者姓名： | 张利娟 沈登荣 张宏瑞 张宏伟 李正跃 |
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| 作者单位： | 1. 云南农业大学农业生物多样性与病虫害控制教育部重点实验室,昆明,650201;云南农业大学植物保护学院,昆明,650201
2. 普洱市宁洱县农业技术推广中心,普洱,665100 |
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| 基金项目： | 农业部公益性行业科研专项项目，云南省自然基金面上项目 |
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| 摘 要： | 在昆虫分子生物学的研究中,从昆虫样品中有效地获得总DNA是分子实验成功的前提。但是,常规提取方法由于不能保留昆虫所有的形态特征,这对于体形较小的珍稀标本是不适用的。文中通过对改进的盐析法和STE法与KAc法的对比,发现盐析法和STE法提取的DNA质量明显优于KAc法,并且能够通过针刺从单头蓟马中成功提取DNA而不影响形态鉴定。2种提取方法的优点是单头蓟马在提取过DNA以后,虫体仍然可用以做成永久玻片进行形态鉴定。提取的DNA经实验证明,可以顺利的进行mtDNA-COI和rDNA-ITS2基因序列引物的扩增。
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| 关 键 词： | 蓟马 DNA提取 PCR |
Method improvement for extraction genomic DNA from thrips |
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| ZHANG Li-Juan,SHEN Deng-Rong,ZHANG Hong-Rui,ZHANG Hong-Wei,LI Zheng-Yue.Method improvement for extraction genomic DNA from thrips[J].Entomological Knowledge,2011,48(3):775-781. |
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| Authors: | ZHANG Li-Juan SHEN Deng-Rong ZHANG Hong-Rui ZHANG Hong-Wei LI Zheng-Yue |
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| Institution: | ZHANG Li-Juan1,2 SHEN Deng-Rong1,2 ZHANG Hong-Rui1,2ZHANG Hong-Wei3 LI Zheng-Yue1,2(1.Ministry of Education Key Laboratory of Agriculture Biodiversity for Plant Disease Management,Kunming 650201,China,2.Plant Protection College,Yunnan Agricultural University,3.Extension Centre of Agricultural Technology,Puer 665100,China) |
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| Abstract: | To isolate total DNA from insect samples is a crucial prerequisite for successful PCR reactions.However,regular methods for isolating insect DNA can't resolve all the morphological characters of insects.Because these methods destroy insects' morphological characteristics they are obviously not suitable for scarce and small insects such as thrips.In this article,two methods of salting-out and the STE method of extracting genome DNA from individual thrips are compared to KAc.The results suggest that,the quali... |
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| Keywords: | PCR |
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