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| 西花蓟马对吡虫啉抗性机制的研究 | |
| 引用本文： | 王圣印,刘永杰,周仙红,张安盛,李丽莉,门兴元,张思聪,于毅.西花蓟马对吡虫啉抗性机制的研究[J].昆虫知识,2011,48(3):559-565. |
| 作者姓名： | 王圣印 刘永杰 周仙红 张安盛 李丽莉 门兴元 张思聪 于毅 |
| 作者单位： | 1. 山东农业大学,泰安,271018;山东省农业科学院植物保护研究所山东省植物病毒学重点实验室,济南,250100 2. 山东农业大学,泰安,271018 3. 山东省农业科学院植物保护研究所山东省植物病毒学重点实验室,济南,250100 |
| 基金项目： | 农业公益性行业科研专项 |
| 摘 要： | 为了解大规模应用吡虫啉防治西花蓟马Frankliniella occidentalis(Pergande)的抗性风险,本文研究了吡虫啉与多杀菌素和啶虫咪的交互抗性并比较了多功能氧化酶系(mixed-function oxidases,MFO)的3个组分细胞色素P450、细胞色素b5、甲氧试卤灵O脱甲基酶(methoxyresorufin O-demethylase,MROD)在西花蓟马敏感品系(S)、抗吡虫啉品系(BK)和北京田间品系(BJ)中的含量以及活性。生物测定表明北京田间品系(BJ)对多杀菌素已经产生了较高抗性(RR50=35.38),而对啶虫咪(RR50=3.05)和吡虫啉(RR50=1.82)处于敏感,交互抗性研究结果表明西花蓟马对吡虫啉与多杀菌素(RR50=1.31)和啶虫咪(RR50=3.20)无交互抗性。生化研究表明:西花蓟马抗吡虫啉品系的3个组分含量均显著高于S品系西花蓟马的含量(P<0.05),其中细胞色素P450在是S品系WFT的4.5倍,细胞色素b5含量是S品系WFT的4.23倍,MROD活性是S品系WFT的5.99倍,表明多功能氧化酶系与西花蓟马对吡虫啉的抗药性密切相关。 |
| 关 键 词： | 西花蓟马 抗药性 细胞色素P450 细胞色素b5 甲氧试卤灵-O-脱甲基酶 |
Mechanisms of imidacloprid resistance in Frankliniella occidentalis |
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| WANG Sheng-Yin,LIU Yong-Jie,ZHOU Xian-Hong,ZHANG An-Sheng,LI Li-Li,MEN Xing-Yuan,ZHANG Si-Cong,YU Yi.Mechanisms of imidacloprid resistance in Frankliniella occidentalis[J].Entomological Knowledge,2011,48(3):559-565. | |
| Authors: | WANG Sheng-Yin LIU Yong-Jie ZHOU Xian-Hong ZHANG An-Sheng LI Li-Li MEN Xing-Yuan ZHANG Si-Cong YU Yi |
| Institution: | WANG Sheng-Yin 1 LIU Yong-Jie1 ZHOU Xian-Hong2 ZHANG An-Sheng2 LI Li-Li2MEN Xing-Yuan2 ZHANG Si-Cong2 YU Yi2(1.Shandong Agricultural University,Taian 271018,China,2.Shandong Academy of Agricultural Sciences,Key Laboratory for Plant Virology of Shandong,Jinan 250100,China) |
| Abstract: | Imidacloprid is a key insecticide universally used for control of the western flower thrip (WFT),Frankliniella occidentalis (Pergande).Resistance to imidacloprid had been studied previously in laboratory selected strains in other insects.These studies mainly attribute imidacloprid resistance to enhanced P450,b5 and methoxy resorufin O-demethylase (MROD) monooxygenases detoxification.Further studies revealed that imidacloprid resistant hoppers did not show cross-resistance to acetamiprid and spinosad insecti... |
| Keywords: | Frankliniella occidentalis insecticide resistance cytochrome P450 cytochrome b5 methoxy resorufin O-demethylase |
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