| 一种经济高效提取实蝇基因组DNA的方法 |
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| 引用本文： | 杨燕,白永华,王绍君,周剑,李克海,丁元明.一种经济高效提取实蝇基因组DNA的方法[J].昆虫知识,2010,47(3):600-603. |
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| 作者姓名： | 杨燕 白永华 王绍君 周剑 李克海 丁元明 |
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| 作者单位： | 1. 云南农业大学,云南,650201
2. 云南出入境检验检疫局,云南,650228 |
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| 基金项目： | 国家质量监督检验检疫局重点项目 |
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| 摘 要： | 单头实蝇高质量基因组DNA的获得为实蝇分子生态学研究奠定了重要基础。本文提出一种经济高效的实蝇基因组DNA提取方法,该方法广泛适用于不同虫态、不同保存条件的实蝇标本,与传统的CTAB法和SDS法相比操作简单、耗时短,得率高。
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| 关 键 词： | 基因组DNA 实蝇 提取 |
An effective and economical method for extracting genomic DNA from the fruit fly |
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| YANG Yan,BAI Yong-Hua,WANG Shao-Jun,ZHOU Jian,LI Ke-Hai,DING Yuan-Ming.An effective and economical method for extracting genomic DNA from the fruit fly[J].Entomological Knowledge,2010,47(3):600-603. |
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| Authors: | YANG Yan BAI Yong-Hua WANG Shao-Jun ZHOU Jian LI Ke-Hai DING Yuan-Ming |
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| Institution: | 1.Yunnan Agricultural University,Kunming 650201,China;2.Yunnan Exit-Entry Inspection and Quarantine Bureau,Kunming 650228,China) |
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| Abstract: | An effective method for extracting genomic DNA from individual specimens is important for the study of molecular phylogenitics in the fruit fly.This paper presented a method for extracting genomic DNA from individual fruit fly.The results showed that the genomic DNA could be successfully extracted from adult,larval and pupal of fruit flies.Meanwhile,this method is also suitable for dried specimens or specimens preserved in alcohol.Compared with traditional methods like CTAB and SDS,it is pretty simple,time saving and effective. |
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| Keywords: | genomic DNA fruit fly extraction |
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