| 不同细菌对家蝇幼虫抗菌蛋白/肽的诱导效应 |
| |
| 引用本文： | 侯利霞,翟培,施用晖,乐国伟.不同细菌对家蝇幼虫抗菌蛋白/肽的诱导效应[J].昆虫知识,2006,43(6):827-831. |
| |
| 作者姓名： | 侯利霞 翟培 施用晖 乐国伟 |
| |
| 作者单位： | 1. 江南大学教育部食品科学和安全重点实验室,无锡,214036
2. 江南大学教育部食品科学和安全重点实验室,无锡,214036;江南大学食品学院食品营养与安全研究所,无锡,214036 |
| |
| 基金项目： | 中国中心研究院所基础科学技术专项基金(No.2001DEA20022)资助 |
| |
| 摘 要： | 利用抑菌圈测量法和毛细管电泳研究、比较不同细菌对家蝇Musca domesticaL.幼虫抗菌蛋白/肽的诱导效应。结果表明,家蝇幼虫受细菌诱导后抗菌活性比对照都有不同程度的增加,同时不同细菌诱导表达样品对于相应的诱导菌均表现很高的抗菌活性。毛细管电泳图谱表明鼠伤寒沙门氏菌Salmonella typhimurium50013诱导后抗菌蛋白/肽表达强度增加了50倍,其它细菌诱导后抗菌蛋白/肽表达强度增加了1～40倍。与G+细菌相比,G-细菌具有更强的诱导效应。结论:家蝇幼虫对不同的细菌刺激有特异性反应,即不同细菌诱导抗菌蛋白/肽的强度、种类和数量都不一致。
|
| 关 键 词： | 家蝇幼虫 抗菌肽 诱导 毛细管电泳 |
| 收稿时间： | 04 27 2006 12:00AM |
| 修稿时间： | 2006-04-272006-09-03 |
Induced responses on the antibacterial proteins/peptides in the larvae of housefly by different bacteria |
| |
| HOU Li-Xia,ZHAI Pei,SHI Yong-Hui,LE Guo-Wei.Induced responses on the antibacterial proteins/peptides in the larvae of housefly by different bacteria[J].Entomological Knowledge,2006,43(6):827-831. |
| |
| Authors: | HOU Li-Xia ZHAI Pei SHI Yong-Hui LE Guo-Wei |
| |
| Institution: | 1. Key Laboratory of Food Science and Safety, Ministry of Education, Southern Yangtze University, Wuxi 214036, China;2. School of Food Science and Technology, Southern Yangtze University, Wuxi 214036, China |
| |
| Abstract: | Inducing effects of different bacteria on the antibacterial proteins/peptides in the larvae of housefly were studied by inhibiting zone determination and capillary electrophoresis.The results suggested that the extracts of the housefly larvae induced by bacteria showed the higher antibacterial activity than CK(non-induced),especially when the respective bacteria was used to induce the larvae.The capillary electrophoresis proteinograms indicated that the expression of antibacterial proteins/peptides was increased by 50 folds when induced by Salmonella typhimurium 50013,while 1～40 folds when induced by the other bacteria.The G-bacteria had a greater potential in induction effeciency than G+.It comes to the conclusion that the housefly larvae respond specifically to the bacterial induction,and produce antibacterial peptides/proteins of various amount and kinds when induced by different bacteria. |
| |
| Keywords: | larvae of housefly(Musca domestica) antibacterial peptide induction capillary electrophoresis |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
|
