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槲皮素对B型烟粉虱羧酸酯酶和谷胱甘肽S-转移酶活性的影响
引用本文:牟少飞,梁沛,高希武.槲皮素对B型烟粉虱羧酸酯酶和谷胱甘肽S-转移酶活性的影响[J].昆虫知识,2006,43(4):491-495.
作者姓名:牟少飞  梁沛  高希武
作者单位:中国农业大学昆虫学系,北京,100094
基金项目:国家973项目资助(2002CB111407)。
摘    要:采用人工饲料添加法,研究植物防御性次生物质槲皮素对B型烟粉虱Bemisiatabaci(Gennadius)成虫主要解毒酶系羧酸酯酶(CarE)和谷胱甘肽S-转移酶(GSTs)影响的剂量效应和时间效应。用低剂量槲皮素(0.01%,wv)处理B型烟粉虱成虫24h后,其羧酸酯酶(CarE)和谷胱甘肽S-转移酶(GSTs)比活力分别为51.09mOD(min·头)和2.249OD(mgpro.min),是对照的1.233倍和2.20倍;而高剂量的槲皮素对2种解毒酶没有诱导增加作用,甚至还有抑制作用。低剂量的槲皮素短时间处理烟粉虱后,可诱导2种酶活性增加,谷胱甘肽S-转移酶(GSTs)在0.005%的槲皮素处理30min后,比活力值达到8.454OD(mgpro·min),为对照的8.30倍。

关 键 词:槲皮素  烟粉虱  羧酸酯酶  谷胱甘肽S-转移酶
收稿时间:03 1 2006 12:00AM
修稿时间:2006-03-012006-04-07

Effects of quercetin on specific activity of carboxylesterase and glutathione S-transferases in Bemisia tabaci
MU Shao-Fei,LIANG Pei,GAO Xi-Wu.Effects of quercetin on specific activity of carboxylesterase and glutathione S-transferases in Bemisia tabaci[J].Entomological Knowledge,2006,43(4):491-495.
Authors:MU Shao-Fei  LIANG Pei  GAO Xi-Wu
Abstract:The effects of quercetin on the specific activity of carboxylesterase(CarE)and glutathione S-transferases(GSTs)in the tobacco whitefly, Bemisia tabaci fed on artificial diet with quercetin were investigated. The results showed that the effects of quercetin on CarE and GSTs specific activity were dependent on dosage and time. Low dosage of treatment with quercetin induced high level of CarE and GSTs specific activity, while high dosage of treatment depressed CarE and GSTs activity. When tobacco whiteflies were treated with 0.01% quercetin, the specific activity of CarE and GSTs were 51.09 mOD/(min·fly) and 2.249 OD/(mg pro·min), respectively. After 30 minutes treatment with 0.005% quercetin, the specific activity of GSTs was 8.454 OD/(mg pro·min).
Keywords:quercetin  bemisia tabaci  carboxylesterase  glutathione S-transferase
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