转基因抗棉铃虫抗草甘膦棉花对棉铃虫的抗虫性及对二种地老虎幼虫生长发育的影响

为了明确转Bt Cry1A+CP4EPSPS基因抗棉铃虫抗草甘膦棉花对棉铃虫Helicoverpa armigera(Hübner)的抗性水平及对八字地老虎Amathes c-nigrum和黄地老虎Agrotis segetum的影响,作者用转基因棉花和非转基因棉花叶片对棉铃虫、八字地老虎和黄地老虎幼虫进行了室内饲养观察。结果表明,转基因抗棉铃虫抗草甘膦棉花对新疆南部第2代棉铃虫表现为中抗,幼虫校正死亡率为70.4%;棉花叶片在整个生育期均能表达Bt毒蛋白,整个生育期Bt毒蛋白含量呈逐渐降低的趋势。转基因抗棉铃虫抗草甘膦棉花对八字地老虎幼虫有强烈的毒杀作用,可以引起幼虫大量死亡,对存活幼虫的生长发育也有明显的抑制作用,幼虫发育迟缓,不能顺利化蛹;但对黄地老虎幼虫没有明显的影响,幼虫可以正常生长。

Resistance of transgenic cotton to cotton bollworm and cutworms

The purpose of this study was to assay the resistance of transgenic cotton expressing Bt Cry1A and CP4 EPSPS genes to the cotton bollworm Helicoverpa armigera （Htibner） and the cutworms Amathes c-nigrum and Agrotis segetum. Results show that transgenic cotton had moderate resistance to early instar second generation cotton bollworm larvae in southern Xinjiang with a corrected mortality of 70.4%. Bt Cry1A toxin protein was present in leaves throughout the entire developmental period of cotton plants but the amounts of this toxin peaked during the cotyledon period and decreased gradually over time. Transgenic cotton was very toxic to A. c-nigrum larvae;feeding on transgenic cotton leaves led to death of most larvae and the growth of surviving larvae was retarded. However, transgenic cotton had no adverse effect on A. segetum larvae which developed normally.